Novel Endpoints for measuring the Toxicity of Ambient PM2.5

Development of Newer Endpoints for measuring the Oxidative Potential of Ambient PM2.5

Measuring DTT consumption in the DTT assay has been the traditional endpoint in PM oxidative potential measurement since more than a decade. However, in our research group’s first study (Xiong et al., 2017), we showed that although DTT oxidation by ambient PM correlates well with H2O2 generation, an overall capability of the ambient aerosols to generate ROS cannot be generalized by this endpoint. For example, H2O2 can further interact with the organic compounds and metals to generate OH radicals, which is not correlated with the DTT oxidation. Thus, the traditional endpoint (DTT consumption) is limited in terms of capturing the interactions among the PM components for ROS generation. To overcome this limitation, we developed a newer endpoint for measuring the OH measurement in DTT assay.

Related Publications

Xiong, Q., Yu, H., Wang, R., Wei, J., Verma, V., 2017. Rethinking dithiothreitol-based particulate matter oxidative potential: measuring dithiothreitol consumption versus reactive oxygen species generation. Environmental Science and Technology, 51, 6507-6514.