PUBLICATIONS Google Scholar h-index = 112 47,468 total citations April 27, 2022
| 393 | Liu, Y., Denisov, I.G., Gregory, M.C., Sligar, S.G. and Kincaid, J.R. (2022) “Importance of Asparagine 202 in Manipulating the Active Site Structure and Preference for Human CYP17A1” Biochemistry 61, 583-594. PMID 35287432. |
| 392 | Liu, Y., Grinkova, Y., Gregory, M.C., Denisov, I.G., Kincaid, J.R. and Sligar, S.G. (2021) “Mechanism of the Clinically Relevant E305G Mutation in Human CYP17A1” Biochemistry 60, 3262-3271. PMC8822902. |
| 391 | Davydov, D.R., Jung, C., Petsko, G.A., Sligar, S.G. and Kornblatt, J.A. (2021) “A Pathfinder in High-Pressure Bioscience: In Memoriam of Gaston Hui Bon Hoa” Biology 10, 778. PMC8389544. |
| 390 | Denisov, I.G., Grinkova, Y.V., Camp, T., McLean, M.A. and Sligar, S.G. (2021) “Midazolam as a Probe for Drug-Drug Interactions Mediated by CYP3A4: Homotropic Allosteric Mechanism of Site-Specific Hydroxylation” Biochemistry 60, 1670-1681. PMC8857940. |
| 389 | Liu, Y., Denisov, I.G., Sligar, S.G. and Kincaid, J.R. (2021) “Substrate-specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b5” J. American Chemical Society 143, 3729-3733. PMC8848836. |
| 388 | Sligar, S.G. and Denisov, I.G. (2020) “Nanodiscs: A Toolkit for Membrane Protein Science” Protein Science 30, 297-315. PMC7784751. |
| 387 | McLean, M.A., Denisov, I.G., Grinkova, Y.V. and Sligar, S.G. (2020) “Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein investigations” Analytical Biochemistry 607, 113860. PMC7492454. |
| 386 | Liu, Y., Denisov, I.G., Grinkova, Y.V., Sligar, S.G. and Kincaid, J.R. (2020) “P450 CYP17A1 Variant with a Disordered Proton Shuttle Assembly Retains Peroxo-Mediated Lyase Efficiency” Chemistry 26, 16846-16852. PMC8848859. |
| 385 | Camp, T. and Sligar, S.G. (2020) “Nanodisc Self-Assembly is Thermodynamically Reversible and Controllable” Soft Matter 16, 5615-5623. PMC7338007. |
| 384 | Gorfe, A. and Sligar, S.G. (2020) “Membrane Bound Ras as a Conformational Clock” Biophysical Journal 118, 1-3. PMC7063474. |
| 383 | Denisov, I.G., Schuler, M.A. and Sligar, S.G. (2019) “Nanodiscs as a New Tool to Examine Lipid-Protein Interactions.” In Kleinschmidt J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology 2003, 645-671. PMC4201044 |
| 382 | Camp, T., Mehta, K., Sligar, S.G. and Zhang, K. (2020) “Molecular Orientation Determination in Nanodiscs at the Single Molecule Level” Analytical Chemistry 92, 2229-2236. PMC7318422 |
| 381 | McLean, M.A., Stephen, A.G. and Sligar, S.G. (2019) “PIP2 Influences the Conformational Dynamics of Membrane Bound KRAS4b” Biochemistry 58, 3537-3545. PMC6447434. |
| 380 | Ivanova, L., Denisov, I.G., Grinkova, Y.V., Sligar, S.G. and Faeste, C.K. (2019) “Biotransformation of the Mycotoxin Enniatin B1 by CYP P4503A4 and Potential for Drug-Drug Interactions” Metabolites 9, 158-175. PMC6724072. |
| 379 | Ghazarian, H., Hu, W., Mao, A., Nguyen, T., Vaidehi, N., Sligar, S.G. and Shively, J.E. (2019) “NMR Analysis of Free and Lipid Nanodisc Anchored CEACAM1 Membrane Proximal Peptides with Ca(2+)/CaM” Biochem. Biophys. Acta Biomembranes 1861, 787-797. PMC6524640 |
| 378 | Camp, T., McLean, M., Kato, M., Cheruzel, L. and Sligar, S. G. (2019) “The Hydrodynamic Motion of Nanodiscs” Physics and Chemistry of Lipids, 220, 28-35. |
| 377 | Denisov, I.G., Grinkova, Y., Nandigrami, P., Shekhar, M., Tajkhorshid, E. and Sligar, S.G. (2019) “Allosteric Interactions in Human Cytochrome P450 CYP3A4: The Role of Phenylalanine 213” Biochemistry 58, 1411-1422. PMC7003538 |
| 376 | Wong, P., Li, L., Chea, J., Poku, E., Ebner, T. Bowkes, N., Wong, J. Y. C., Yazaki, P. J., Sligar, S. G., and Shively, J. E. (2020) “Antibody targeted PET Imaging of 64Cu-DOTA-Anti-CEA PEGylated Lipid Nanodiscs in CEA positive tumors” Bioconjugate Chemistry 31, 743-753. |
| 375 | Mustafa, G., Nandekar, P.P., Camp, T.J., Bruce, N.J., Gregory, M.C., Sligar, S.G. and Wade, R.C. (2019) “Influence of Transmembrane Helix Mutations on Cytochrome P450-Membrane Interactions and Function” Biophysical Journal 116, 1-14. PMC6369400 |
| 374 | Bu, R.L., Clark, J., DiChiara, T., Sligar, S.G. and Klein, W.L. (2018) “SMPL Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Biology of the Synapses.” Springer Nature Neuromethods 141, Synaptosomes (Murphy, K. M., ed.) pp. 227-250. |
| 373 | Duggal, R., Denisov, I.G. and Sligar, S.G. (2018) “Cytochrome b5 Enhances Androgen Synthesis by Rapidly Reducing the CYP17A1 Oxy-complex in the Lyase Step” FEBS Letters 592, 2282-2288. PMC6369587 |
| 372 | Mak, P.J., Duggal, R., Denisov, I.G., Gregory, M.C. and Sligar, S.G. (2018) “Human Cytochrome CYP17A1: The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone” J. American Chemical Society 140, 7324-7331. PMC5999583 |
| 371 | McLean, M. A., Gregory, M.C. and Sligar, S. G. (2018) “Nanodiscs: A Controlled Bilayer Surface for the study of Membrane Proteins” Annual Reviews Biophysics 47, 107-124. PMC6370528 |
| 370 | Gregory, M.C., Mak, P.J., Khatri, Y., Kincaid, J.R. and Sligar, S.G. (2018) “Human CYP17A1: Control of Substrate Preference by Asparagine 202.” Biochemistry 57, 764-771. PMC5801141. |
| 369 | Denisov, I.G., Baylon, J.L., Grinkova, Y.V., Tajkhorshid, E. and Sligar, S.G. (2018) “Drug-Drug Interactions Between Atorvastatin and Dronedarone Mediated by Monomeric CYP3A4” Biochemistry 57, 805-816. PMC5800941. |
| 368 | Mak, P. J. and Denisov, I. G. (Sligar lab) (2018) “Spectroscopic Studies of the Cytochrome P450 Reaction Mechanism.” BBA Proteins and Proteomics 1866, 178-204. PMC5709052 |
| 367 | Wade J.H., Jones J.D., Lenov I.L., Riordan C.M., Sligar S.G. and Bailey R.C. (2017) “Microfluidic Platform for Efficient Nanodisc Assembly, Membrane Protein Incorporation and Purification”. Lab Chip 22, 2951-2959. PMC5589448. |
| 366c | Guo et al. (2017) Correction Cell Chemical Biology 24, 1314. |
| 366 | Guo Z., Sevrioukova, I.F., Denisov, I.G., Zhang, X., Chiu, T.L., Thomas, D.G., Hanse, E.A., Cuellar, R.A.D., Grinkova, Y.V., Langenfeld, V.W., Swedien, D.S., Stamschror, J.D., Alvarez, J., Luna, F., Galván, A., Bae, Y.K., Wulfkuhle, J.D., Gallagher, R.I., Petricoin, E.F. Rd, Norris, B., Flory, C.M., Schumacher, R.J., O’Sullivan, M.G., Cao, Q., Chu, H., Lipscomb, J.D., Atkins, W.M., Gupta, K., Kelekar, A., Blair, I.A., Capdevila, J.H., Falck, J.R., Sligar, S.G., Poulos, T.L., Georg, G.I., Ambrose, E., Potter, D.A. (2017) “Heme binding bi-guanidines target cytochrome P450 dependent cancer cell mitochondria” Cell Chemical Biology, 24, 1259-1275. PMC5650512.
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| 365 | DiChiara, T., DiNunno, N., Clark, J., Ru, R., Cline, E.N., Rollins, M.G., Gong, Y., Brody, D.L., Sligar, S.G., Velasco, P.T., Viola, K.L., and Klein, W. L. (2017) “Alzheimer’s Toxic Amyloid Beta Oligomers: Unwelcome Visitors to the Na/K ATPase alpha3 Docking Station”. Yale Journal of Biology and Medicine 90, 45-61. PMC5369044. |
| 364 | Gregory, M.C., McLean, M.A. and Sligar, S.G. (2017) “Interaction of KRas4b with Anionic Membranes: A Special Role for PiP2” Biochemical and Biophysical Research Communications 487, 351-355. PMC5509366. |
| 363 | Van Eps, N., Caro, L.N., Morizumi, T., Kusnetzow, A.K., Szczepek, M., Hoffman, K.P., Bayburt, T.H., Sligar, S.G., Ernst, O.P. and Hubbell, W. L. (2017) “Conformational Equilibria of Light Activated Rhodopsin in Nanodiscs” Proc. Natl. Acad. Sci. USA 17, 2951-2959. PMC5402410. |
| 362 | Denisov, I.G. and Sligar, S.G. (2017) “Nanodiscs in Membrane Biochemistry and Biophysics” Chemical Reviews 117, 4669-4713. PMC5805400. |
| 361 | Ye, X., McLean, M.A. and Sligar, S. G. (2016) “PIP2 Modulates the Affinity of Talin-1 for Phospholipid Bilayers and Activates its Auto-Inhibited Form”. Biochemistry 55, 5038-5048. PMC5808563 |
| 360 | Her, C., Filoti, D.I., McLean, M.A., Sligar, S.G., Ross, J.B.A., Steel, H. and Laue, T.M. (2016) “The Charge Properties of Phospholipid Nanodiscs” Biophysical Journal 111, 989-998. PMC5018125 |
| 359 | Ye, X., McLean, M.A. and Sligar, S.G., (2016) “Conformational Equilibrium of Talin is Regulated by Anionic Lipids.” Biochemica et Biophysica Acta 1858, 1833-1840. PMC5671925 |
| 358 | Duggal, R., Liu, Y., Gregory, M., Denisov, I.D., Kincaid, J. and Sligar, S. G. (2016) “Evidence that Cytochrome b5 Acts as a Redox Donor in CYP17A1 Mediated Androgen Biosynthesis.” Biochemical and Biophysical Research Communications 477, 202-208. PMC4935565 |
| 357 | Denisov, I.G. and Sligar, S.G. (2016) “Nanodiscs for Structural and Functional Studies of Membrane Proteins.” Nature Structural and Molecular Biology 23, 481-486. PMC8934039 |
| 356 | Reichart, T. M., Baksh, M. M., Rhee, J.K., Fiedler, J. D., Sligar, S. G., Finn, M. G., Zwick, M. B. and Dawson P. E. (2016) “Trimerization of the HIV Transmembrane Domain in Lipid Bilayers Modulates Broadly Neutralizing Antibody Binding.” Angewandte Chemie Int. Edition 55, 2688-2692. PMC5405556 |
| 355 | Denisov, I.G., Mak, P.J., Grinkova, Y.V., Bastien, D., Berube, G., Sligar, S.G. and Kincaid, J.R. (2016) “The Use of Isomeric Testosterone Dimers to Explore Allosteric Effects in Substrate Binding to Cytochrome P450 CYP3A4”, J. Inorganic Biochemistry 158, 77-85. PMC4887402 |
| 354 | Mak, P.J., Gregory, M.C., Denisov, I.G., Sligar, S.G. and Kincaid, J.R. (2015) “Unveiling the Crucial Intermediates in Androgen Production” Proceedings National Academy of Sciences USA 112 (52), 15856-15861. PMC4703009 |
| 353 | Skar-Gislinge, N., Kynde, S.A.R., Denisov, I. G., Ye, X., Lenov, I., Sligar, S.G., and Arleth, L. (2015) “Small Angle Scattering Determination of the Shape and Localization of Human Cytochrome P450 Embedded in a Phospholipid Nanodisc Environment.” Acta Cryst. D71, 2412-2421. PMC4667284 |
| 352 | Denisov, I. G., and Sligar, S. G. (2015) “Metalloproteins: The Long and Short Of It.” Nature Chemistry News & Views, 7(9), 687-688. PMID: 26291939 |
| 351 | Wilcox, K. C., Marunde, M., Das, A., Velasco, P., Kuhns, B., Marty, M., Jiang, H., Lacor, P., Luan, C-H, Sligar, S. G., and Klein, W. L. (2015) “Nanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen that Targets Binding Sites for Alzheimer’s-Associated Aß Oligomers.” PLoS One 10(4), 1 (April 30) PMC4415972. |
| 350 | Carney, C. E., Lenov, I. L., Baker, C. J., MacRenaris, K. W., Eckermann, A. L., Sligar, S. G., and Meade, T. J. (2015) “Nanodiscs as a Modular Platform for Multimodal MR-Optical Imaging.” Bioconjugate Chemistry 26, 899-905. PMC4486023 |
| 349 | Denisov, I. G., Sligar, S. G. (2015) “Activation of Molecular Oxygen in Cytochromes P450.” In: Cytochrome P450: Structure, Mechanism and Biochemistry, 4th ed. P. R. Ortiz de Montellano Ed., Springer International Publishing, Heidelbert-New York, p. 69-109. |
| 348 | Denisov, I. G., Grinkova, Y. V., Baylon, J. L., Tajkorshid, E., and Sligar S. G. (2015) “Mechanism of Drug-Drug Interactions Mediated by Human Cytochrome P450 CYP3A4 Monomer,” Biochemistry, 54, 2227-2239. PMC4757451 |
| 347 | Khatri, Y., Luthra, A., Duggal, R., and Sligar, S. G. (2014) “Kinetic Solvent Isotope Effect in Steady-State Turnover by CYP19A1 Suggests Involvement of Compound 1 For Both Hydroxylation and Aromatization Steps,” FEBS Letters 588,3117-3122. PMC4138292 |
| 346 | Mak, P.J., Luthra, A., Sligar, S. G., and Kincaid, J. R. (2014) “Resonance Raman Spectroscopy of the Oxygenated Intermediates of Human CYP19A1 Implicates a Compound 1 Intermediate in the Final Lyase Step,” J. Am. Chem. Soc. 136, 4825-4828. PMC3985783 |
| 345 | Khatri, Y., Gregory, M.C., Grinkova, Y.V., Denisov, I.G., and Sligar, S.G. (2014) “Active Site Proton Delivery and the Lysase Activity Human CYP17A1.” Biochem. Biophys. Res. Commun. 443, 179-184. PMC3912944 |
| 344 | D’Antona, A.M., Xie, G., Sligar, S.G., and Oprian, D.D. (2014) “Assembly of an Activated Rhodopsin/Transduction Complex in Nanoscale Lipid Bilayers.” Biochemistry 53, 127-134. PMC3939051 |
| 343 | Mak, P.J., Gregory, M.C., Sligar, S.G., and Kincaid, J.R. (2014) “Resonance Raman Spectroscopy Reveals that Substrate Structure Selectively Impacts the Heme-Bound Diatomic Ligands of CYP17.” Biochemistry 53, 90-200. PMC3922198 |
| 342 | Marty, M.T., Zhang, H., Cui, W., Gross, M.L., and Sligar, S.G. (2014) “Interpretation and Deconvolution of Nanodisc Native Mass Spectra.” J. Am. Soc. Mass. Spectrom. 25, 269-277 PMC3918181 |
| 341 | Gregory, M.C., Denisov, I.G., Grinkova, Y.V., Khatri, Y., and Sligar, S.G. (2013) “Kinetic Solvent Isotope Effect in Human P450 CYP17A1-Mediated Androgen Formation: Evidence for a Reactive Peroxoanion Intermediate.” J. Am. Chem. Soc. 135, 16245-16247. PMC3867208 |
| 340 | Sun, Y., Zeng, W., Benabbas, A., Ye, X., Denisov, I., Sligar, S.G., Du, J., Dawson, J.H., and Champion, P.M. (2013) “Investigations of Heme Ligation and Ligand Switching in Cytochromes P450 and P420. Biochemistry 52, 5941-5951 PMC3777526 |
| 339 | Vishnivetskiy, S.A., Ostermaier, M.K., Singhal, A., Panneels, V., Homan, K.T., Glukhova, A., Sligar, S.G., Tesmer, J.J., Schertler, G.F., Standfull, J., and Gurevich, V.V. (2013) “Constitutively Active Rhodopsin Mutants Causing Night Blindness are Effectively Phosphorylated by GRKs but Differ in Arrestin-1 Binding.” Cell Signal. 25(11): 2155-2162. PMC774132 |
| 338 | Baylon, J.L., Lenov, I., Sligar, S.G., and Tajkhorshid, E. (2013) “Characterizing the Membrane-Bound State of Cytochrome P450 3A4: Structure, Depth of Insertion, and Orientation.” J. Am. Chem. Soc. 135, 8542-8551. PMC3682445 |
| 337 | Luthra, A., Gregory, M., Grinkova, Y. V., Denisov, I. G., Sligar, S. G. (2013) “Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes.” Methods Mol. Biol., 987, 115-127. PMC23475672 |
| 336 | Numata, M., Grinkova, Y.V., Mitchell, J.R., Chu, H.W., Sligar, S.G., and Voelker, D.R. (2013) “Nanodiscs as a Therapeutic Delivery Agent: Inhibition of Respiratory Syncytial Virus Infection in the Lung.” International Journal of Nanomedicine 8, 1417-1427. PMC3663477 |
| 335 | Gregory, M., Mak, P.J., Sligar, S. G., and Kincaid, J.R. (2013) “Differential Hydrogen Bonding in Human CYP17 Dictates Hydroxylation Versus Lyase Chemistry.” Angewandte Chemie, Int. Ed. 52, 5342-5345. PMC3772656 |
| 334 | Sloan, C.D.K., Marty, M.T., Sligar, S.G., and Bailey, R.C. (2013) “Interfacing Lipid Bilayer Nanodiscs and Silicon Photonic Sensor Arrays for Multiplexed Protein-Lipid and Protein-Membrane Protein Interaction Screening.” Analytical Chemistry 85, 2970-2976. PMC3600637 |
| 333 | Marty, M. T., Wilcox, K. C., Klein, W. L., and Sligar, S.G. (2013) “Nanodisc-Solubilized Membrane Protein Library Reflects the Membrane Proteome.” Anal. Bioanal. Chem. 405, 4009-4016. PMC3628400 |
| 332 | Schuler, M. A., Denisov, I. G., and Sligar, S. G. (2013) “Nanodiscs as a New Tool to Examine Lipid-Protein Interactions.” Methods Mol. Biol. 974, 415-433. PMC4201044 |
| 331
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Grinkova, Y.V., Denisov, I.G., McLean, M.A., and Sligar, S.G. (2013) Oxidase Uncoupling in Heme Monooxygenases: Human Cytochrome P450 CYP3A4 in Nanodiscs.” Biochem. Biophys. Res. Comm. 430, 1223-1227. PMC4191626 |
| 330 | Marty, M.T., Zhang, H., Cui, W., Blankenship, R.E., Gross, M.L. and Sligar, S.G. (2012) “Native Mass Spectrometry Characterization of Intact Nanodisc Lipoprotein Complexes.” Analytical Chemistry 84, 8957-8960. PMC3536499 |
| 329 | Denisov, I.G., Grinkova, Y.V., and Sligar, S.G (2012) “Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450” Methods Mol. Biol., 875, 375-391. PMC4196337 |
| 328 | Marty, M.T., Kuhnline Sloan, C. D., Bailey, R.C., Sligar, S.G., (2012) “Nonlinear Analyte Concentration Gradients for One –Step Kinetic Analysis Employing Optical Microring Resonators.” Analytical Chemistry 84, 5556-5564. PMC3428216 |
| 327 | Dalal, K., Chan, C. S., Sligar, S. G., and Duong, F. (2012) “Two Copies of the SecY Channel and Acidic Lipids are Necessary to Activate the SecA Translocation ATPase.” Proceedings of the National Academy of Sciences USA 109, 4104-4109. PMC3306715 |
| 326 | Morrissey, J. H., Tajkhorshid, E., Sligar, S. G., and Rienstra, C. M., (2012) “Tissue Factor/Factor VIIa Complex: Role of the Membrane Surface.” Thrombosis Research 129 Suppl. 2, S8-S10. PMC3335922 |
| 325 | Ojemyr, L. N., con Ballmoos, C., Gennis, R. B., Sligar, S. G., and Brzezinski, P. (2012) “Reconstitution of Respiratory Oxidases in Membrane Nanodiscs for Investigation of Proton-Coupled Electron Transfer.” FEBS Letters 586, 640-645. PMC4103190 |
| 324 | Denisov, I. G., and Sligar, S. G. (2012) “A Novel Type of Allosteric Regulation: Functional Cooperativity in Monomeric Proteins.” Archives Biochemistry Biophysics 519, 91-102. PMC3329180 |
| 323 | Marty, M. T., Das, A., and Sligar, S.G. (2012) “Ultra-Thin Layer MALDI Mass Spectrometry of Membrane Proteins in Nanodiscs.” Analytical and Bioanalytical Chemistry 402, 721-729.
PMC3249468 |
| 322 | Denisov, I. G., Shih, A. Y., and Sligar, S. G. (2012) “Structural Differences Between Soluble and Membrane Bound Cytochrome P450s.” J. Inorganic Biochemistry 108, 150-158. PMC4190058 |
| 321 | de la Rama, L. P., Hu, L., Efremov, M. Y., Olson, E. A., Nealey, P. F., McLean, M. A., Sligar, S. G., and Allen, L. A. (2011) “Anomalous Transitions of DODAB Using Fast Scanning Liquid Calorimetry.” Thermochimica Acta 522, 72-76. |
| 320 | Goldsmith, B.R., Mitali, J.J., Josue, J., Castro, A., Lerner, M.B., Bayburt, T.H., Khamis, S.M., Jones, R.A., Brand, J.G., Sligar, S.G., Luetje, C.W., Gelperin, A., Rhodes, P.A., Discher, B.M., Johnson, A.T. (2011) “Biomimetic Chemical Sensors Using Nanoelectronic Readout of Olfactory Receptor Proteins.” ACS Nano 5, 5408-5416. PMC4165341 |
| 319
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Luthra, A., Denisov, I.G., Sligar, S.G., (2011) “Temperature Derivative Spectroscopy to Monitor the Autoxidation Decay of Cytochromes P450.” Analytical Chemistry 83, 5394-5399. PMC3163897 |
| 318 | Karunakaran, V., Denisov, I., Sligar, S. G., and Champion, P. M. (2011) “Investigation of the Low Frequency Dynamics of Heme Proteins: Native and Mutant Cytochrome P450cam and Redox Partner Complexes.” J. Physical Chemistry B 115, 5665-5677 PMC3088772 |
| 317 | Mak, P. J., Denisov, I. G., Grinkova, Y. V., Sligar, S. G., Kincaid, J. R. (2011) “Defining CYP3A4 Structural Responses to Substrate Binding. Raman Spectroscopic Studies of a Nanodisc-Incorporated Mammalian Cytochrome P450.” J. Am. Chem. Soc. 133, 1357-1366. PMC3033474 |
| 316 | Luthra, A., Denisov, I. G., Sligar. S. G. (2011) “Spectroscopic Features of Cytochrome P450 Reaction Intermediates.” Archives Biochemistry Biophysics 507, 26-25. PMC3041835 |
| 315 | Sligar, S. G. (2010) “Chemistry. Glimpsing the Critical Intermediate in Cytochrome P450 Oxidations.” Science 330, 924-925. PMC4103181 |
| 314 | Grinkova Y.V., Denisov I.G., Sligar S.G. (2010) “Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers.” Protein Engineering Design and Selection 23, 843-848. PMC2953958 |
| 313
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Frank. D. J., Denisov, I. G., Sligar, S. G. (2010) “Analysis of Heterotropic Cooperativity in Cytochrome P450 3A4 Using α-Naphthoflavone and Testosterone.” J. Biological Chemistry 286, 5540-5545. PMC2953958 |
| 312 | Bayburt, Timothy H., Vishnivetskiy, Sergey A., McLean, Mark A., Morizumi, Takefumi, Huang, Chih-chin, Tesmer, John J. G., Ernest, Oliver, P., Sligar, Stephen G., and Gurevich, Vsevolod, V. (2010) “Monomeric Rhodopsin is Sufficient for Normal Rhodopsin Kinase (GRK1) Phosphorylation and Arrestin-1 Binding.” J. Biol. Chem. 286, 1420-1428. PMC3020750 |
| 311 | Ham, M-H., Choi, J. H., Boghossian, A. A., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S. G., Wraight, C. A., and Strano, M. S. (2010) “Photoelectrochemical Complexes for Solar Energy Conversion that Chemically and Autonomously Regenerate.” Nature Chemistry 2, 929-936. PMC4103191 |
| 310 | Skar-Gislinge, N., Simonsen, J. B., Mortensen, K., Feidenhans’l, R., Sligar, S. G., Møller, B. L., Bjørnholm, T., and Arleth, L. (2010) “Elliptical Structure of Phospholipid Bilayer Nanodiscs Encapsulated by Scaffold Proteins: Casting the Roles of the Lipids and the Protein.” J. Am. Chem. Soc. 132, 13713-13722. PMC4120756 |
| 309 | Tark, S-H, Das, A., Sligar, S., and Dravid, V. P. (2010) “Nanomechanical Detection of Cholera Toxin Using Microcantilevers Functionalized With Ganglioside Nanodiscs.” Nanotechnology 21, 435502. PMC3868204 |
| 308 | Denisov, I.G., and Sligar, S.G., (2010) “Cytochromes P450 in Nanodiscs.” Biochem. Biophys. Acta. 1814, 223-229. PMC2974961 |
| 307 | Grashoff, C., Hoffman, B. D., Brenner, M. D., Zhou, R., Parsons, M., Yang, M. T., McLean, M. A., Sligar, S. G., Chen, C. S., Ha, T., and Schwartz, M. A. (2010) “Measuring Mechanical Tension Across Vinculin Reveals Regulation of Focal Adhesion Dynamics.” Nature 466, 263-266. PMC2901888 |
| 306 | Grinkova, Y.V., Denisov, I.G, and Sligar, S.G., (2010) “Functional Reconstitution of Monomeric CYP3A4 with Multiple Cytochrome P450 Reductase Molecules in Nanodiscs.” Biochem. Biophys. Res. Comm. 398, 194-198. PMC4103192 |
| 305 | Ye, F., Hu, G., Taylor, D., Ratnikov, B., Bobkov, A.A., McLean, M.A., Sligar, S.G., Taylor, K.A., Ginsberg, M.H. (2010) “Recreation of the Terminal Events in Physiological Integrin Activation.” J. Cell Biol. 188, 157-173. PMC2812850 |
| 304 | Kijac, A., Shih, A., Nieuwkoop, A., Sligar, S. G., and Rienstra, C. (2010) “Lipid-Protein Correlations in Nanoscale Phospholipid Bilayers by Solid-State Nuclear Magnetic Resonance.” Biochemistry 2, 9190-9198. PMC3136391 |
| 303 | Davydov, D.R., Sineva, E.V., Sistla, S., Davydova, N.Y., Frank, D.J., Sligar, S.G., and Halpert, J.R. (2009) “Electron Transfer in the Complex of Membrane-Bound Human Cytochrome P450 3A4 with the Flavin Domain of P450BM-3: The Effect of Oligomerization of the Heme Protein and Intermittent Modulation of the Spin Equilibrium.” Biochim. Biophys. Acta. 1797, 378-390. PMC2819549 |
| 302 | Fruh, V., Zhou, Y., Chen, D., Loch, C., Eiso, S.B., Grinkova, Y.N., Verheij, H., Sligar, S.G., Bushweller, J.H., and Siegal, G. (2010) “Application of Fragment-Based Drug Discovery to Membrane Proteins: Identification of Inhibitors of the Integral Membrane Enzyme DsbB.” Chemistry & Biology 17, 881-891. PMC4104428 |
| 301 | Das, A. and Sligar, S.G. (2009) “Modulation of the Cytochrome P450 Reductase Redox Potential by the Phospholipid Bilayer.” Biochemistry 48, 12104-12112. PMC2797566 |
| 300 | Ritchie, T.K., Grinkova, Y.V., Bayburt, T.H., Denisov, I.G., Zolnerciks, J.K., Atkins, W.M., and Sligar, S.G. (2009) “Reconstitution of Membrane Proteins in Phospholipid Bilayer Nanodiscs.” Methods in Enzymology 464, 211-231. PMC4196316 |
| 299 | Bayburt, T. H., and Sligar, S. G. (2010) “Membrane Protein Assembly into Nanodiscs.” FEBS Letters 584, 1721-1727. PMC4758813 |
| 298 | Dalal, K., Nguyen, N., Alami, M., Tan, J., Moraes, T. F., Lee, W. C., Maurus, R., Sligar, S. G., Brayer, G. D., and Duong, F. (2009) “Structure, Binding and Activity of Syd, a SecY-Interacting Protein.” J. Biol. Chem. 284, 7897-7902. PMC2658082 |
| 297 | Gantt, S. L., Denisov, I. G., Grinkova, Y. V., and Sligar, S. G. (2009) “The Critical Iron-Oxygen Intermediate in Human Aromatase.” Biochem. Biophys. Res. Comm. 387, 169-173. PMC2753498 |
| 296 | Morrissey, J. H., Pureza, V., Davis-Harrison, R. L., Sligar, S. G., Rienstra, C. M., Kijac, A. Z., Ohkubo, Y. Z., and Tajkhorshid, E. (2009) “Protein-Membrane Interactions: Blood Clotting on Nanoscale Bilayers.” J. Thrombosis and Haemostasis 7, 169-172. PMC2839880 |
| 295 | Frank, D. J., Denisov, I. G., and Sligar, S. G. (2009) “Mixing Apples and Oranges: Analysis of Heterotropic Cooperativity in Cytochrome P450 3A4.” Archives Biochemistry and Biophysics 488, 146-152.
PMC4752175 |
| 294 | Shih, A.Y. Sligar, S. G., and Schulten, K. (2009) “Maturation of High-Density Lipoproteins.” J. Royal Society Interface 6, 863-871. PMC2805102 |
| 293 | Denisov, I. G., Frank, D. J., and Sligar, S. G. (2009) “Cooperative Properties of Cytochromes P450.” Pharmacology & Therapeutics 124, 151-167. PMC2753496 |
| 292 | Das, A., Zhao, J., Schatz, G. C., Sligar, S. G., and Van Duyne, R. P. (2009) “Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy.” Anal. Chem. 81, 3754-3759. PMC4757437 |
| 291 | McLean, M. A., Rajfur, S., Chen, Z., Humphrey, D., Yang, B., Sligar, S.G., and Jacobson, K. (2009) “Mechanism of Chromophore Assisted Laser Inactivation Employing Fluorescent Proteins.” Anal. Chem. 81, 1755-1761. PMC2865575 |
| 290 | Li, H., Das, A., Sibhatu, H., Jamal, J., Sligar, S.G., and Poulos, T.L. (2008) “Exploring the Electron Transfer Properties of Neuronal Nitric Oxide Synthase by Reversal of the FMN Redox Potential.” J. Biol. Chem. 283, 34762-34772. PMC2596388 |
| 289 | Shih, A.Y., Freddolino, P.L., Arkhipov, A., Sligar, S.G., and Schulten, K. (2008) “Molecular Modeling of the Structural Properties and Formation of High-Density Lipoprotein Particles.” Current Topics in Membranes 60, 313-342. |
| 288 | Goluch, E.D., Shaw, A. W., Sligar, S.G,, and Liu, C. (2008) “Microfluidic Patterning of Nanodisc Lipid Bilayers and Multiplexed Analysis of Protein Interaction.” Lab Chip 8, 1723-1728. PMID: 18813396 |
| 287 | Zhao, J., Das, A., Schatz, G.C., Sligar, S.G., and VanDuyne, R. P. (2008) “Resonance Localized Surface Plasmon Spectroscoy: Sensing Substrate and Inhibitor Binding to Cytochrome P450.” J. Phys. Chem. 112, 13084-13088. |
| 286 | Borch, J., Torta, F., Sligar, S. G., and Roepstorff, P. (2008) “Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor.” Analytical Chemistry 80, 6245-6252. PMID: 18616345 |
| 285 | Denisov, I. G., Mak, P. J., Makris, T. M., Sligar, S. G., and Kincaid, J. R. (2008) “Resonance Raman Characterization of the Peroxo and Hydroperoxo Intermediates in Cytochrome P450.” Journal of Physical Chemistry A 112, 13172-13179. PMC3120136 |
| 284 | Kuhnel, K., Ke, N., Cryle, M. J., Sligar, S. G., Schuler, M. A., and Schlichting, I (2008) “Crystal Structures of Substrate-Free and Retinoic Acid-Bound Cyanobacterial Cytochrome P450 CYP120A1.” Biochemistry 47 6552-6559. PMID: 18512957 |
| 283 | Grinkova, Y.V., Denisov, I. G., Waterman, M. R., Arase, M., Kagawa, N., and Sligar, S. G. (2008) “The Ferrous-Oxy Complex of Human Aromatase.” Biochem. Biophys. Res. Comm. 372, 379-382. PMC2478517 |
| 282 | Morrissey, J. H., Pureza, V., Davis-Harrison, R. L., Sligar, S. G., Ohkubo, Y. Z. and Tajkhorshid, E. (2008) “Blood Clotting Reactions on Nanoscale Phospholipid Bilayers.” Thrombosis Research 122 (Supplement 1), S23-S26. PMC2836762 |
| 281 | Gruia, F., Ionascu, D., Kubo, M., Ye, X., Dawson, J., Osborne, R.L., Sligar, S.G., Denisov, I., Das, A., Poulos, T.L., Terner, J., and Champion, P.M. (2008) “Low Frequency Dynamics of Caldariomyces Fumago Chloroperoxidase Probed by Femtosecond Coherence Spectroscopy.” Biochemistry 47, 5156-5167. PMC2766418 |
| 280 | Shih, A.Y., Sligar, S.G., and Schulten, K. (2008) “Molecular Models Need to be Tested: The Case of the Solar Flares Discoidal HDL Model.” Biophysical Journal 94, L87-L89. PMC2397337 |
| 279 | Newcomb, M., Halgrimson, J.A., Horner, J.H., Wasinger, E.C., Chen, L.X., and Sligar, S.G. (2008) “X-Ray Absorption Spectroscopic Characterization of a Cytochrome P450 Compound II Derivative.” Proceedings of the National Academy of Sciences 24, 8179-8184. PMC2448811 |
| 278 | Hall, B., McLean, M. A., Davis, K., Casanova, J. E., Sligar, S. G., and Schwartz, M. A. (2008) “A Fluorescence Resonance Energy Transfer Activation Sensor for Arf6.” Analytical Biochemistry 374, 243-249. PMC2277471 |
| 277 | Makris, T.M., vonKoenig, J., Schlichting, I., Sligar, S.G. (2007) “Alteration of P450 Distal Pocket Solvent Leads to Impaired Proton Delivery and Changes in Heme Geometry.” Biochemistry 46, 14129-14140.
PMID: 18001135 |
| 276 | Davydov, D.R., Baas, B.J., Sligar, S.G., Haplert, J.R. (2007) “Allosteric Mechanisms in Cytochrome P450 3A4 Studied by High-Pressure Spectroscopy: Pivotal Role of Substrate-Induced Changes in the Accessibility and Degree of Hydration of the Heme Pocket.” Biochemistry 46, 7852-7864. PMC2527461 |
| 275 | Marin, V. L., Bayburt, T. H., Sligar, S. G., and Mrksich, M. (2007) “Functional Assays of Membrane-Bound Proteins with SAMDI-TOF Mass Spectrometry.” Angew. Chem, Int. Ed. 46, 8796-8798. PMC2790068 |
| 274 | Kijac, A.Z., Li, Y., Sligar, S.G., and Rienstra, C.M. (2007) “Magic-Angle Spinning Solid-State NMR Spectroscopy of Nanodisc-Embedded Human CYP3A4.” Biochemistry 46, 13696-13703. PMC2571072 |
| 273 | Das, A., Grinkova, Y.V., and Sligar, S.G. (2007) “Redox Potential Control by Drug Binding to Cytochrome P450 3A4.” J. Am. Chem. Soc. 129, 13778-13779. PMC2535932 |
| 272 | Denisov, I.G., Dawson, J.H., Hager, L.P., and Sligar, S.G. (2007) “The Ferric-Hydroperoxo Complex of Chloroperoxidase.” Archives Biochemistry and Biophysics 363, 954-958. PMC2078203 |
| 271 | Shih, A.Y., Arkhipov, A., Freddolino, P.L., Sligar, S.G. and Schulten, K. (2007) “Assembly of Lipids and Proteins into Lipoprotein Particles.” J. Phys. Chem. B 111, 11095-11104. PMID: 17696388 |
| 270 | Sligar, S. G., and Denisov. I. G. (2007) “Understanding Cooperativity in Human P450 Mediated Drug-Drug Interactions.” Drug Metabolism Reviews 39, 567-579. PMID: 17786639 |
| 269 | Denisov, I.G., Grinkova, Y.V., McLean, M.A., and Sligar, S. G. (2007) “The One Electron Autoxidation of Human Cytochrome P4503A4.” J. Biol. Chem. 282, 26865-26873. PMID: 17650504 |
| 268 | Kimmich, N., Das, A., Sevrioukova, I., Meharenna, Y., Sligar, S. G., and Poulos, T. L. (2007) “Electron Transfer Between Cytochrome P450CIN and It’s FMN-Containing Redox Partner, Cindoxin.” J. Biol. Chemistry, 282, 27006-27011. PMID 17606612 |
| 267 | Nath, A., Grinkova, Y.V., Sligar, S. G., and Atkins, W. M. (2007) “Ligand Binding to Cytochrome P450 3A4 in Phospholipid Bilayer Nanodiscs: The Effect of Model Membranes.” J Biol. Chem. 282, 28309-28320. PMID: 17573349 |
| 266 | Mak, P.J., Denisov, I.G., Victoria, D., Makris, T.M., Deng, T., Sligar, S.G., and Kincaid, J.R. (2007) “Resonance Raman Detection of the Hydroperoxo Intermediate in the Cytochrome P450 Enzymatic Cycle.” J. Am. Chem. Soc. 129, 6382-6383. PMC2574425 |
| 265 | Zhao, Q., Sigalov, G., Dimitrov, V., Dorvel, B., Mirsaidov, U., Sligar, S., Aksimentiev, A. and Timp, G. (2007) “Detecting SNPs Using a Synthetic Nanopore.” NanoLetters, 7, 1680-1685. PMC2565804 |
| 264 | Shih, A. Y., Freddolino, P. L., Sligar, S. G. and Schulten, K. (2007) “Disassembly of Nanodiscs with Cholate.” NanoLetters, 7, 1692-1696. PMID: 17503871 |
| 263 | Bayburt, T.H., Leitz, A.J., Xie, G., Oprian, D.D., and Sligar, S.G. (2007) “Transducin Activation by Nanoscale Lipid Bilayers Containing One and Two Rhodopsins.” J. Biol. Chem. 282, 14875-14881. PMID: 17395586 |
| 262 | Chougnet, A., Grinkova, Y., Ricard, D., Sligar, S., and Woggon, W-D (2007) “Fluorescent Probes for Raid Screening of Potential Drug-Drug Interactions at the CYP3A4 Level.” Chem. Med. Chem. 2, 717-724. PMID: 17357170 |
| 261 | Alami, M., Dalal, K., Lelj-Garolla, B., Sligar, S. G., and Duong, F. (2007) “Nanodiscs Unravel the Interaction Between the SecYEG Channel and it’s Cytosolic Partner SecA.” The EMBO Journal 26, 1995-2004.
PMC1852787 |
| 260 | Nath, A., Atkins, W. M., and Sligar, S. G. (2007) “Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins.” Biochemistry 46, 2059-2069. PMID: 17263563 |
| 259 | Denisov, I.G., Baas, B.J., Grinkova, Y.V., and Sligar, S. G. (2007) “Cooperativity in P450 CYP3A4: Linkages in Substrate Binding, Spin State, Uncoupling and Product Formation.” J. Biol. Chem. 282, 7066-7076. PMID: 17213193 |
| 258 | Denisov, I.G., Victoria, D.C., Sligar, S.G. (2007) “Cryoradiolytic Reduction of Heme Proteins: Maximizing Dose-Dependent Yield.” Radiation Physics and Chemistry 76, 714-721. PMC2131698 |
| 257 | Shaw, A.W., Pureza, V.S., Sligar, S.G., and Morrissey, J.H. (2007) “The Local Phospholipid Environment Modulates the Activation of Blood Clotting.” J. Biol. Chem. 282, 6556-6563. PMID: 17200119 |
| 256 | Li, Y., Kijac, A. Z., Sligar, S. G., and Rienstra, C. M. (2006) “Structural Analysis of Nanoscale Self-Assembled Discoidal Lipid Bilayers by Solid-State NMR Spectroscopy.” Biophys. J. 91, 3819-3828. PMC1630456 |
| 255 | Zhao, J., Das, A., Zhang, X., Schatz, G. C., Sligar, S. G., and Van Duyne, R. P. (2006) “Resonance Surface Plasmon Spectroscopy: Low molecular Weight Substrate Binding to Cytochrome P450.” J. A. Chem. Soc. 128, 11004-11005. PMID: 16925400 |
| 254 | Boldog, T., Grimme, S., Li, M., Sligar, S. G., and Hazelbauer, G. L. (2006) “Nanodiscs Separate Chemoreceptor Oligomeric States and Reveal Their Signaling Properties.” Proceedings of the National Academy of Sciences 103, 11509-11514. PMC1544200 |
| 253 | Newcomb. M., Zhang, R., Chandrasena, R.E.P., Halgrimson, J. A., Horner, J. H., Makris, T. M., and Sligar, S. G. (2006) “Cytochrome P450 Compound l.” J. American Chemical Society 128, 4580-4581. PMC2536593 |
| 252 | Bayburt, T. H., Grinkova, Y. V., and Sligar, S. G., (2006) “Assembly of Single Bacterorhodopsin Trimers in Bilayer Nanodiscs.” Archives Biochemistry and Biophysics 450, 215-222. PMID: 16620766 |
| 251 | Denisov, I. G., Grinkova, Y.V., Baas, B.J., and Sligar, S.G. (2006) “The Ferrous-Dioxygen Intermediate in Human Cytochrome P450 3A4: Substrate Dependence of Formation and Decay Kinetics.” J. Biol. Chem, 281, 23313-23318. PMID: 16762915 |
| 250 | Leitz, A. J., Bayburt, T. H., Barnakov, A. N., Springer, B. A., and Sligar, S. G. (2006) “Functional Reconstitution of ß2-Adrenergic Receptors Utilizing Self-Assembling Nanodisc Technology.” BioTechniques 40, 601-610. PMID: 16708760 |
| 249 | Makris, T. M., von Koenig, K., Schlichting, I. and Sligar, S. G. (2006) “The Status of High-Valent Metal Oxo Complexes in the P450 Cytochromes.” J. Inorganic Biochemistry 100, 507-518. PMID: 16510191 |
| 248 | Heng, J. B., Aksimentiev, A., Ho, C., Grinkova, Y. V., Sligar, S. G., Schulten, K., and Timp, G. (2006) “The Electromechanics of DNA in a Synthetic Nanopore.” Biophysical Journal 90, 1098-1106. PMC1367096 |
| 247 | Heng, J. B., Aksimentiev, A., Ho, C., Marks, P., Grinkova, Y. V., Sligar, S. G. and Schulten, K. (2005) “Stretching DNA Using the Electric Field in a Synthetic Nanopore.” NanoLetters 5, 1883-1888. PMC2441849 |
| 246 | Davydov, Dmitri, Fernando, Harshica, Baas, Bradley J., Sligar, Stephen G., and Halpert, James R. (2005)”Kinetics of Dithionite-Dependent Reduction of Cytochrome P450 3A4: Heterogeneity of the Enzyme Caused by It’s Oligomerization.” Biochemistry 44, 13902-13913. PMC1343486 |
| 245 | Sligar, Stephen G., Makris, Thomas M., and Denisov, Ilia G. (2005) “Thirty Years of Microbial P450 Monooxygenase Research: Peroxo-Heme Intermediates–The Central Bus Station in Heme Oxygenase Catalysis.” Biochem. Biophys. Res. Comm. 338, 346-354. PMID: 16139790 |
| 244 | Denisov, I. G., Makris, Thomas M., Sligar, Stephen G., and Schlichting, Ilme (2005) “Structure and Chemistry of Cytochrome P450.” Chemical Reviews 105, 2253-2277. PMID: 15941214 |
| 243 | Denisov, I. G., McLean, M. A., Shaw, A. W., Grinkova, Y. V., and Sligar, S. G. (2005) “Thermotropic Phase Transition in Soluble Nanoscale Lipid Bilayers.” J. Phys. Chem. 109, 15580-15588. PMC2518645 |
| 242 | Ke, N., Baudry, J., Makris, T. M., Schuler, M. A., and Sligar, S. G. (2005) “A Retinoic Acid Binding Cytochrome P450: CYP120A1 from Synechocystis sp PCC 6803.” Archives Biochemistry and Biophysics 436, 110-120. PMID: 15752715 |
| 241 | Sono, M., Perera, R., Jin, S., Makris, T. M., Sligar, S. G., Bryson, T. A., and Dawson, J. H. (2005) “The Influence of Substrate on the Spectral Properties of Oxyferrous Wild-Type and T252A Cytochrome P450-CAM.” Archives Biochemistry and Biophysics 436, 40-49. PMID: 15752707 |
| 240 | Shih, A. Y., Denisov, I. G., Phillips, J. C., Sligar, S. G., Schulten, K. (2005) “Molecular Dynamics Simulations of Discoidal Bilayers Assembled from Truncated Human Lipoproteins.” Biophysical Journal 88, 548-556. PMID: 15533924 PMCID: PMC1305032 |
| 239 | Schunemann, V., Lendzian, F., Jung, C., Contzen, J., Barra, A. L., Sligar, S. G., Trautwein, A. X. (2004) “Tyrosine Radical Formation in the Reaction of Wild Type and Mutant Cytochrome 450cam with Peroxy Acids: A Multifrequency EPR Study of Intermediates on the Millisecond Time Scale.” J. Biol. Chem 279, 10919-10930. PMID: 14688245 |
| 238 | Heng, J.B., Ho, C., Kim, T., Timp, R., Aksimentiev, A., Grinkova, Y.V., Sligar, S.G., Schulten, K., and Timp, G. (2004) “Sizing DNA Using a Nanometer-Diameter Pore.” Biophysical Journal 87, 2905-2911.
PMID: 15326034 PMCID: PMC1304707 |
| 237 | Baas, B. J., Denisov, I. G., and Sligar, S. G. (2004) “Homotropic Cooperativity of Monomeric Cytochrome P450 3A4 in a Nanoscale Native Bilayer Environment.” Archives Biochemistry and Biophysics 430, 218-228. PMID: 15369821 |
| 236 | Duan, H., Civjan, N., Sligar, S. G. and Schuler, M. A. (2004) “Co-incorporation of Heterologously-Expressed Arabidopsis Cytochrome P450 and P450 Reductase into Soluble Nanoscale Lipid Bilayers” Archives Biochemistry and Biophysics 424, 141-153. PMID: 15047186 |
| 235 | Denisov, I.G., Grinkova, Y.V., Lazarides, A.A., and Sligar, S.G. (2004) “Directed Self-Assembly of Monodisperse Phospholipid Bilayer Nanodiscs With Controlled Size.” J. American Chemical Society 126, 3477-3487. PMID: 15025475 |
| 234 | Shaw, A.W., McLean, M.A., Sligar, S.G. (2004) “Phospholipid Phase Transitions in Homogeneous Nanometer Scale Bilayer Discs.” FEBS Letters 556, 260-264. PMID: 14706860 |
| 233 | Sligar, Stephen G. (2003) “Finding a Single-Molecule Solution for Membrane Proteins.” Biochem. Biophys. Res. Comm. 312, 115-119. PMID: 14630028 |
| 232 | Ibrahim, M., Denisov, I. G., Makris, T. M., Kincaid, J. R., and Sligar, S. G. (2003) “Resonance Raman Spectroscopic Studies of Hydroperoxo-Myoglobin at Cryogenic Temperatures.” J. Am. Chem. Soc. 125, 13714-13718. PMID: 14599210 |
| 231 | Bayburt, T. H., and Sligar, S. G. (2003) “Self- Assembly of Single Integral Membrane Proteins into Soluble Nanoscale Phospholipid Bilayers.” Protein Science 12, 2476-2481. PMID: 14573860 PMCID: PMC2366955 |
| 230 | Huisman, G., and Sligar, S. G. (2003) “Evolution of Protein Technologies from Voodoo to Science,” Editorial Overview, Current Opinion in Biotechnology 14, 357-359. PMID: 12943842 |
| 229 | Civjan, N. R., Bayburt, T. H., Schuler, M. A., and Sligar, S. G. (2003) “Direct Solubilization of Heterologously Expressed Membrane Proteins by Incorporation into Nanoscale Lipid Bilayers.” BioTechniques 35, 556-563. PMID: 14513561 |
| 228 | Makris, T. M., Denisov, I. G., and Sligar, S. G. (2003) “Haem-Oxygen Reactive Intermediates: Catalysis by the Two-Step.” Biochemical Society Transactions 31, 516-519. PMID: 12773147 |
| 227 | Jin, S. X., Makris, T.M., Bryson, T. A., Sligar, S. G., and Dawson, J. H. (2003) “Epoxidation of Olefins by Hydroperoxo-Ferric Cytochrome P450.” J. Am. Chem. Soc. 125, 3406-3407. PMID: 12643683 |
| 226 | Dmochowski, I. J., Dunn, A. R., Wilker, J. J., Crane, B. R., Green, M. T., Dawson, J. H., Sligar. S. G., Winkler, J. R., and Gray, H. B. (2002) “Sensitizer-Linked Substrates and Ligands: Ruthenium Probes of Cytochrome P450 Structure and Mechanism. “ Methods in Enzymology, 120-133. PMID: 12424904 |
| 225 | Denisov, I. G., Ikeda-Saito, M., Yoshida, T., and Sligar, S. G. (2002) “Cryogenic Absorption Spectra of Hydroperoxo-Ferric Heme Oxygenase, the Active Intermediate of Enzymatic Heme Oxygenation.” FEBS Letters 532, 203-206. PMID: 1245949 |
| 224 | Denisov, I. G., Makris, T. M., and Sligar, S. G. (2002) “Formation and Decay of Hydroperoxo-Ferric Heme Complex in Horseradish Peroxidase Studied by Cryoradiolysis.” J. Biol. Chem. 277, 42706-42710. PMID: 12215454 |
| 223 | Makris, T. M., Davydov, R., Denisov, I. G., Hoffman, B. M., and Sligar, S. G. (2002) “Mechanistic Enzymology of Oxygen Activation by the Cytochromes P450.” Drug Metabolism Reviews 34, 691-708. PMID: 12487147 |
| 222 | Park, S. Y., Yamane, K., Adachi, S., Shiro, Y., Maves, S. A., Weiss, K. E., and Sligar, S. G. (2002) “Thermophilic Cytochrome P450 (CYP119) from Sulfolobus Solfataricus: High Resolution Structural Origin of its Thermostability and Functional Properties.” J. Inorganic Biochemistry 91, 491-501. PMID: 12237217 |
| 221 | Hoa, G. H. B., McLean, M. A., and Sligar, S. G. (2002) “High Pressure, A Tool for Exploring Heme Protein Active Sites (Review).” Biochimica et Biophysica Acta 1595, 297-308. PMID: 11983404 |
| 220 | Bayburt, T. H., Grinkova, Y. V., and Sligar, S. G. (2002) “Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins.” NanoLetters 2, 853-856. |
| 219 | Sanders, K. E., Lo, J., and Sligar, S. G. (2002) “Intersubunit Circular Permutation of Human Hemoglobin.” Blood Journal 100, 299-305. PMID: 12070040 |
| 218 | Lynch, T. W., and Sligar, S. G. (2002) “Experimental and Theoretical High Pressure Strategies for Investigating Protein-Nucleic Acid Assemblies.” Biochimica et Biophysica Acta 1595, 277-282. PMID: 11983402 |
| 217 | Bayburt, T. H., and Sligar, S. G. (2002) “Single Molecule Height Measurements on Microsomal Cytochrome P450in Nanometer-Scale Phospholipid Bilayer Disks.” Proceedings of the National Academy of Sciences 99, 6725-6730. PMID: 11997441 PMCID: PMC124470 |
| 216 | Kellner, D. G., Hung, S-C., Weiss, K. E., and Sligar, S. G. (2002) “Kinetic Characterization of Compound I Formation in the Thermostable Cytochrome P450 CYP119.” J. Biol. Chem. 277, 9641-9644. PMID: 11799104 |
| 215 | Unno, M., Christian, J. F., Sjodin, T., Benson, D. E., Macdonald, I. D. G., Sligar, S. G., and Champion, P. M. (2002) “Complex Formation of Cytochrome P450cam with Putidaredoxin, Evidence for Protein-Specific Interactions Involving the Proximal Thiolate Ligand.” J. Biol. Chem. 277, 2547-2553. PMID: 11706033 |
| 214 | Lynch, T. W., Kosztin, D., McLean, M. A., Schulten, K., and Sligar, S. G. (2002) “Dissecting the Molecular Origins of protein-Nucleic Acid Recognition: High Pressure and Molecular Dynamics.” Biophysical Journal 82, 93-98. PMID: 11751298 PMCID: PMC1302451 |
| 213 | Rosca, F., Kuman, A.T.N., Ionascu, D., Ye, X., Demidov, A. A., Sjodin, T., Wharton, D., Barrick, D., Sligar, S. G., Yonetani, T., and Champion, P. M. (2002) “Investigations of Anharmonic Low Frequency Oscillations in Heme Proteins.” J. Phys. Chem. 106, 3540-3552. |
| 212 | Denisov, I. G., Hung, S. C., Weiss, K. E., McLean, M. A., Shiro, Y., Park, S. Y., Champion, P. M., and Sligar, S. G. (2001) “Characterization of the Oxygenated Intermediate of the Thermophilic Cytochrome P450 CYP 119.” J. Inorganic Biochemistry 87, 215-226. PMID: 11744059 |
| 211 | Ivanov, Y. D., Kanaeva, I. P., Karuzina, I. I., Usanov, S. A., Hui Bon Hoa, G., Sligar, S. G., and Archakov. A. I., (2001) “Revelation of Ternary Comlexes between Redox Partners in Cytochrome P450-Containing Monooxygenase Systems by the Optical Biosensor Method.” J. Inorganic Biochemistry87, 175-184.
PMID: 11437357 |
| 210 | Sjodin, T., Christian, J. F., Davydov, R., Unno, M., Macdonald, I.D.G., Sligar, S. G., Hoffman, B. M., and Champion, P. M. (2001) “Resonance Raman and EPR Investigations of D251N Oxycytochrome P450cam/Putidaredoxin Complex.” Biochemistry, 40, 6852-6859. PMID: 11389599 |
| 209 | Ivanov, Y. D., Kanaeva, I. P., Karuzine, I. I., Archakov, A. I., Hui Bon Hoa, G., and Sligar, S. G. (2001) “Molecular Recognition in the P450cam Monoxygenase System: Direct Monitoring of Protein-Protein Interactions by Using Optical Biosensor.” Archives Biochem. Biophys. 391, 255-264. |
| 208 | Denisov, I. G., Makris, T. M., and Sligar, S. G. (2001) “Cryotrapped Reaction Intermediates of Cytochrome P450 Studied by Radiolytic Reduction with Phosphorus-32.” J. Biol. Chem., 276, 11648-11652. PMID: 11152470 |
| 207 | Davydov, R., Makris, T. M., Kofman, V., Werst, D. E., Sligar, S. G., and Hoffman, B. M. (2001) “Hydroxylation of Camphor by Reduced Oxy-Cytochrome P450cam: Mechanistic Implications of EPR and ENDOR Studies of Catalytic Intermediates in Native and Mutant Enzymes.” J. Am. Chem. Soc., 123, 1403-1415. PMID: 11456714 |
| 206 | Maves, S. A., and Sligar, S. G. (2001) “Understanding Thermostability in Cytochrome P450 by Combinatorial Mutagenesis.” Protein Science, 10, 161-168. PMID: 11266604 PMCID: PMC2249849 |
| 205 | Deng, T. J., Macdonald, I. D. G., Simianu, M., Sykora, M., Kincaid, J. R., and Sligar S. G. (2001) “Hydrogen-Bonding Interactions in the Active Sites of Cytochrome P450cam and Its Site-Directed Mutants.” J. Am. Chem. Soc., 123, 269-278. PMID: 11456513 |
| 204 | Park, S. Y., Yamane, K., Adachi, S., Shiro, Y., Weiss, K. E., and Sligar, S. G. (2000) “Crystallization and Preliminary X-Ray Diffraction Analysis of a Cytochrome P450 (CYP119) from Sulfolobus solfataricus.” Acta, Cryst. 56, 1173-1175. PMID: 10957637 |
| 203 | Lynch, T. W., and Sligar, S. G. (2000) “Macromolecular Hydration Changes Associated with BamHI Binding and Catalysis.” J. Biological Chemistry, 275, 30561-30565. PMID: 10878000 |
| 202 | Shank-Retzlaff, M.L., and Sligar, S. G. (2000) “Analyte Gradient – Surface Plasmon Resonance: A One-Step Method for Determining Kinetic Rates and Macromolecular Binding Affinities.” Analytical Chemistry, 72, 4212-4220. PMID: 10994986 |
| 201 | Bondos, S. E., Sligar, S. G., and Jonas, J. (2000) “High Pressure Denaturation of Apomyoglobin.” Biochimica Biophysica ACTA, 1480, 353-364. PMID: 11004573 |
| 200 | Bayburt, T. H., Carlson, J. W., and Sligar, S. G. (2000) “Single Molecule Height Measurements on a Membrane Protein in Nanometer-Scale Phospholipid Bilayer Disks.” Langmuir, 16, 5993-5997. PMID: 11997441 |
| 199 | Carlson, J. W., Bayburt, T., and Sligar, S. G. (2000) “Nanopatterning Phospholipid Bilayers.” Langmuir, 16, 3927-3931. |
| 198 | Schlichting, I., Berendzen, J., Chu, K., Stock, A. M., Maves, S. A., Benson, D. E., Sweet, R. M., Ringe, D., Petsko, G. A., and Sligar, S. G. (2000) “The Catalytic Pathway of Cytochrome P450cam at Atomic Resolution.” Science, 287, 1615-1622. PMID: 10698731 |
| 197 | Yeung, C., Purves, T., Kloss, A. A., Kuhl, T. L., Sligar, S. G., and Leckband, D. (1999) “Cytochrome c Recognition of Immobilized, Orientational Variants of Cytochrome b5: Direct Force and Equilibrium Binding Measurements.” Langmuir, 15, 6829-6836. |
| 196 | Davydov, R., Macdonald, I.D.G., Makris, T. M., Sligar, S. G., and Hoffman, B. M. (1999) “EPR and ENDOR of Catalytic Intermediates in Cryoreduced Native and Mutant Oxy-Cytochromes P450cam: Mutation-Induced Changes in the Proton Delivery System.” Journal of the American Chemical Society, 121, 10654-10655. |
| 195 | Deng, T., Proniewicz, L. M., Kincaid, J. R., Yeom, H., Macdonald, I. D. G., and Sligar, S. G. (1999) “Resonance Raman Studies of Cytochrome P450BM3 and Its Complexes with Exogenous Ligands.” Biochemistry, 38, 13699-13706. PMID: 10521277 |
| 194 | Zhu, L., Zhong, G., Unno, M., Sligar, S. G., and Champion, P. M. (1999) “Femtosecond Coherence Spetroscopy of a Heme Protein.” Biospectroscopy, 2, 301-309. |
| 193 | Muller, J. D., McMahon, B. H., Chien, E. Y. T., Sligar, S. G., and Nienhaus, G. U. (1999) “Connection Between the Taxonomic Substates and Protonation of Histidines 64 and 97 in Carbonmonoxy Myoglobin.” Biophysical Journal, 77, 1036-1051. PMID: 10423448 PMCID: PMC1300394 |
| 192 | Carlson, J. W., Godfrey, B. J. and Sligar, S. G. (1999) “Metal Binding Colloidal Gold Particles: A Versatile SFM Tip Calibrator for Fluid Imaging.” Langmuir, 15, 3086-3090. |
| 191 | Macdonald, I.D.G., Sligar, S. G., Christian, J. F., Unno, M., and Champion, P. M. (1999) “Identification of the Fe-O-O Bending Mode in Oxycytochrome P450cam by Resonance Raman Spectroscopy.” Journal of the American Chemical Society, 121, 376-380 |
| 190 | McLean, M. A., Maves, S. A., Weiss, K. E., Krepich, S., and Sligar, S. G. (1998) “Characterization of a Cytochrome P450 from the Acidothermophilic Archaea Sulfolobus solfataricus.” Biochem. Biophys. Res. Comm. 252, 166-172. PMID: 9813164 |
| 189 | Shank-Retzlaff, M. L., Raner, G.M., Coon, M. J., and Sligar, S. G. (1998) “The Membrane Topology of Cytochrome P4502B4 in Langmuir-Blodgett Monolayers.” Archives Biochemistry & Biophysics, 359, 82-88. PMID: 9799564 |
| 188 | Bayburt, T. H., Carlson, J. W., and Sligar, S. G. (1998) “Reconstitution and Imaging of a Membrane Protein in a Nanometer-Sized Phospholipid Bilayer.” Journal of Structural Biology, 123, 37-44. PMID: 9774543 |
| 187 | Peterson, E. S., Friedman, J. M., Chien, E. Y. T., and Sligar, S. G. (1998) “Functional Implications of the Proximal Hydrogen Bonding Network in Myoglobin: A Resonance Raman and Kinetic Study of Leu89, Ser92, His97 and F-Helix Swap Mutants.” Biochemistry, 37, 12301-12319. PMID: 9724545 |
| 186 | Vidakovic, M., Sligar, S. G., Li, H., and Poulos, T. L. (1998) “Understanding the Role of the Essential Asp251 in Cytochrome P450cam Using Site Directed Mutagenesis, Crystallography and Kinetic Solvent Isotope Effect.” Biochemistry, 37, 9211-9219. PMID: 9649301 |
| 185 | Wu, Y., Chien, E. Y.T., Sligar, S. G., and LaMar, G. N. (1998) “Influence of Proximal Side Mutations on the Molecular and Electronic Structure of Cyanomet Myoglobin: A 1H NMR Study.” Biochemistry, 37, 6979-6990. PMID: 9578585 |
| 184 | Robinson, C. R., Liu, Y., O’Brien, R., Sligar, S. G., and Sturtevant, J. M. (1998) “A Differential Scanning Calorimetric Study of the Thermal Unfolding of Apo- and Holo-Cytochrome b562. Protein Science, 7, 961-965. PMID: 9568902 |
| 183 | Kwiatkowski, L. D., Hui, H. L., Wierzba, A., Noble, R. W., Walder, R. Y., Peterson, E. S., Sligar, S. G., and Sanders, K. E. (1998) “Preparation and Kinetic Characterization of a Series of βW37 Variants of Human Hemoglobin A: Evidence for High Affinity T Quaternary Structures.” Biochemistry, 37, 4325-4335.
PMID: 9521753 |
| 182 | Robinson, C. R., and Sligar, S. G. (1998) “Changes in Solvation During DNA Binding and Cleavage are Critical to Altered Specificity of the EcoRI Endonuclease.” Proceedings of the National Academy of Sciences, 95, 2186-2191. PMID: 9482860 |
| 181 | Robinson, C. R., Liu, Y., Thomson, J. A., Sturtevant, J. M., and Sligar, S. G. (1997) “Energetics of Heme Binding to Native and Denatured States of Cytochrome b562.” Biochemistry, 36, 16141-16146.
PMID: 9405047 |
| 180 | Maves, S. A., Yeom, H., McLean, M. A., and Sligar, S. G. (1997) “Decreased Substrate Affinity Upon Alteration of the Substrate-Docking Region in Cytochrome P450BM-3.” FEBS Letters, 414, 213-218.
PMID: 9315688 |
| 179 | Christian, J. E., Unno, M., Sage, J. T., Champion, P. M., Chien, E., and Sligar, S. G. (1997) “Spectroscopic Effects of Polarity and Hydration in the Distal Heme Pocket of Deoxymyoglobin.” Biochemistry, 36, 11198-11204. PMID: 9287162 |
| 178 | Carlson, J. W., Jonas, A., and Sligar, S. G. (1997) “Imaging and Manipulation of High-Density Lipoproteins.” Biophysical Journal, 73, 1184-1189. PMID: 9284285 PMCID: PMC1181017 |
| 177 | Unno, M., Christian, J. F., Benson, D. E., Gerber, N. C., Sligar, S. G., and Champion, P. M. (1997) “Resonance Raman Investigations of Cytochrome P450cam Complexed with Putidaredoxin.” Journal of the American Chemical Society, 119, 6614-6620. |
| 176 | Kellner, D. G., Maves, S. A., and Sligar, S. G. (1997) “Engineering Cytochrome P450s for Bioremediation.” Current Opinion in Biotechnology, 8, 274-278. PMID: 9206006 |
| 175 | Pearson, J. G., Montez, B., Le, H., Oldfield, E., Chien, E.Y., and Sligar, S. G. (1997) “Assignment and Analysis of Fluorine Nuclean Magnetic Resonance Spectra of 4-Fluorotryptophan Myoglobins and Hemoglobins.” Biochemistry, 36, 3590-3599. PMID: 9132010 |
| 174 | Rector, K.D., Rella, C.W., Hill, J.R., Kwok, A., Sligar, S. G., Chien, E.Y.T., Dlott, D. D., and Fayer, M.D. (1997) “Mutant and Wild Type Myoglobin-CO Protein Dynamics: Vibrational Echo Experiments.” Journal of Physical Chemistry, 101, 1468-1475. |
| 173 | Benson, D. E., Suslick, K. S., and Sligar, S. G. (1997) “Reduced Oxy Intermediate Observed in D251N Cytochrome P450cam.” Biochemistry, 36, 5104-5107. PMID: 9136869 |
| 172 | Yeom, H., and Sligar, S. G. (1997) “Oxygen Activation by Cytochrome P450BM-3: Effects of Mutating an Active Site Acidic Residue.” Archives of Biochemistry and Biophysics, 337, 209-216. PMID: 9016815 |
| 171 | DiPrimo, C., Deprez, E., Sligar, S. G., and Hui Bon Hoa, G. (1997) “Origin of the Photoacoustic Signal in Cytochrome P-450cam: Role of the Arg186-Asp251-Lys178 Bifurcated Salt-Bridge,” Biochemistry, 36, 112-118. PMID: 8993324 |
| 170 | McLean, M. A., Yeom, H., and Sligar, S. G. (1996) “Carbon Monoxide Binding to Cytochrome P450BM-3: Evidence for a Substrate Dependent Conformational Change.” Biochimie, 78, 700-705. PMID: 9010598 |
| 169 | Blanke, S. R., Martinis, S. A., Sligar, S. G., Hager, L. P., Rux, J. J., and Dawson, J. H. (1996) “Probing the Heme Iron Coordination Structure of Alkaline Chloroperoxidase.” Biochemistry, 35, 14537-14543.
PMID: 8931550 |
| 168 | Martinis, S. A., Blanke, S. R., Hager, L. P., Sligar, S. G., Hui Bon Hoa, G., Rux, J. J., and Dawson, J. H. (1996) “Probing the Heme Iron Coordination Structure of Pressure-Induced Cytochrome P420cam.” Biochemistry, 35, 14530-14536. PMID: 8931549 |
| 167 | Robinson, C. R., and Sligar, S. G. (1996) “Participation of Water in Hin Recombinase-DNA Recognition.” Protein Science, 5, 2119-2124. PMID: 8897613 PMCID: PMC2143269 |
| 166 | Firestone, M. A., Shank, M. L., Sligar, S. G., and Bohn, P. W. (1996) “Film Architecture in Biomolecular Assemblies. Effect of Linker on the Orientation of Genetically Engineered Surface-Bound Proteins.” Journal of the American Chemical Society, 118, 9033-9041. |
| 165 | Sanders, K.E., Ackers, G., and Sligar, S.G. (1996) “Engineering and Design of Blood Substitutes.” Current Opinion in Structural Biology, 6, 534-540. PMID: 8794165 |
| 164 | Scott, Jill R., Fairris, Jill L., McLean, M., Wang, K. F., Sligar, S. G., Durham, B., and Millett, F. (1996) “Intramolecular Electron-Transfer Reactions of Cytochrome B(5) Covalently Bonded to Ruthenium(II) Polypyridine Complexes – Reorganizational Energy and Pressure Effects.” Inorganica Chimica Acta, 243, 193-200. |
| 163 | Tian, W. D., Sage, J. T., Champion, P. M., Chien, E.Y.T., and Sligar, S. G. (1996) “Probing Heme Protein Conformational Equilibration Rates with Kinetic Selection.” Biochemistry, 35, 3487-3502. PMID: 8639499 |
| 162 | Jung, C., Ristau, O., Schulze, H., and Sligar, S. G. (1996) “The CO Stretching Mode Infrared Spectrum of Substrate-Free Cytochrome P450cam-CO. The Effect of Solvent Conditions, Temperature, and Pressure.” European Journal of Biochemistry, 235, 660-669. PMID: 8654415 |
| 161 | Jiang, M., Nölting, B., Stayton, P. S. and Sligar, S.G. (1996) “Surface-Linked Molecular Monolayers of an Engineered Myoglobin: Structure, Stability and Function.” Langmuir, 12, 1278-1283. |
| 160 | Yeom, H., Sligar, S. G., Poulos, T., and Fulco, A. (1995) “The Role of Thr268 in Oxygen Activation in Cytochrome P-450BM-3.” Biochemistry, 34, 14733-14740. PMID: 7578081 |
| 159 | Hernan, R. A., and Sligar, S. G. (1995) “Tetrameric Hemoglobin Expressed in Escherichia Coli.: Evidence of Heterogeneous Subunit Assembly.” Journal of Biological Chemistry, 270, 26257-26264. PMID: 7592833 |
| 158 | Durham, B., Fairris, J. L., McLean, M., Millett, F., Scott, J. R., Sligar, S. G., and Willie, A. (1995) “Electron Transfer from Cytochrome B5 to Cytochrome C.” J. Bioenergetics and Biomembranes, 27, 331-340. PMID: 8847346 |
| 157 | Dumoulin, A., Kiger, L., Jiang, R., Baudin, V., Vasseur, C., Sligar, S. G., Marden, M. C., Pagnier, J., and Poyart, C. (1995) “Loss of Allosteric Behavior in Recombinant Hemoglobin 2 292(F8) His -> Ala: Restoration Upon Addition of Strong Effectors.” FEBS Letters, 374, 39-41. PMID: 7589508 |
| 156 | McLean, M. A., and Sligar, S. G. (1995) “Thermodynamic Characterization of the Interaction Between Cytochrome b5 and Cytochrome c.” Biochemical and Biophysical Research Communications, 215, 316-320. PMID: 7575608 |
| 155 | Abadan, Y., Chien, E. Y. T., Chu, K., Eng. C. D., Nienhaus, G. U., and Sligar, S. G. (1995) “Ligand Binding to Heme Proteins. V. Light-Induced Relaxation in Proximal Mutants L891 and H97F of Carbonmonoxymyoglobin.” Biophysical Journal, 68, 2497-2504. PMID: 7647252 PMCID: PMC1282159 |
| 154 | Robinson, C. R., and Sligar, S. G. (1995) “Heterogeneity in Molecular Recognition by Restriction Endonucleases: Osmotic and Hydrostatic Pressure Effects on BamHI, PvuII, and EcoRV Specificity.” Proceedings of the National Academy of Sciences, 92, 3444-3448. PMID: 7724581 PMCID: PMC42183 |
| 153 | Loida, P., Paulsen, M., Arnold, G., Ornstein, R., and Sligar, S. G. (1995) “Stereoselective Hydroxylation of Norcamphor by Cytochrome P-450cam: Experimental Verification of Molecular Dynamics Simulations.” The Journal of Biological Chemistry, 270, 5326-5330. PMID: 7890644 |
| 152 | Tian, W. D., Wells, An V., Champion, P. M., Di Primo, C., Gerber, N., and Sligar, S. G. (1995) “Measurements of CO Geminate Recombination in Cytochrome P450 and P420.” The Journal of Biological Chemistry, 270, 8673-8679. PMID: 7721770 |
| 151 | Deprez, E., Gerber, N. C., Di Primo, C., Douzou, P., Sligar, S. G., and Hoa, G., (1994) “Electrostatic Control of the Substrate Access Channel in Cytochrome P-450cam.” Biochemistry 33, 14464-14468. PMID: 7981206 |
| 150 | LaMar, G. N., Dalichow, F., Zhao, X., Dou, Y., Idedo-Saito, M., Chiu, M. L., and Sligar, S. G. (1994) “1HMR Investigation of Distal Mutant Deoxy Myoglobins. Interpretation of Proximal His Contact Shifts in Terms of Localized Distal Water Molecule.” The Journal of Biological Chemistry 269, 29629-29635.
PMID: 7961951 |
| 149 | Pin, S., Albert, B., Cortes, R., Ascone, I., Chiu, M. L., and Sligar, S. G. (1994) “The Heme Iron Coordination Complex in HIS64(E7)TYR Recombinant Sperm Whale Myoglobin.” Biochemistry 33, 11618-11623.
PMID: 7918375 |
| 148 | Scott, J. R., McLean, M., Sligar, S. G., Durham, B., and Millett, F. (1994) “Effect of Binding Cytochrome c and Ionic Strength on the Reorganizational Energy and Intramolecular Electron Transfer in Cytochrome b5 Labeled with Ruthenium(II) Polypyridine Complexes.” Journal of the American Chemical Society 116, 7356-7362. |
| 147 | Theriault, Y., Pochapsky, T. C., Dalvit, C., Chiu, M. L., Sligar, S. G., and Wright, P. E. (1994) “1H and 15N Resonance Assignments for the Carbon Monoxide Complex of Sperm Whale Myoglobin.” J. Biomolec. NMR, 4, 491-504. PMID: 8075538 |
| 146 | Gerber, N. C., and Sligar, S. G. (1994) “A Role for Asp251 in Cytochrome P-450cam Oxygen Activation.” The Journal of Biological Chemistry 269, 4260-4266. PMID: 8307990 |
| 145 | Rajarathnam, K., Qin, J., La Mar, G. N., Chiu, M. L., and Sligar, S. G. (1994) “Correlation Between the Steric Bulk of the Distal E7 and E11 Residues and the Tilt of the Fe-CN Unit in Cyanometmyoglobin as Determined by NMR from the Orientation of the Magnetic Axes in Single and Double Point Mutants.” Biochemistry 33, 5493-5501. PMID: 8180171 |
| 144 | Fann, Y-C., Gerber, N. C., Osmulski, P. A., Hager, L. P., Sligar, S. G., and Hoffman, B. M. (1994) “ENDOR Determination of Heme Ligation in Choloroperoxidase and Comparison with Cytochrome P-450cam.” Journal of the American Chemical Society 116, 5989-5990. |
| 143 | Springer, B. A., Sligar, S. G., Phillips, G. N., and Olson, J. S. (1994) “Mechanisms of Ligand Recognition in Myoglobin.” Chem. Rev. 94, 699-714. |
| 142 | Banci, L., and Sligar, S. G. (1994) “Cytochrome P450 and Aromatic Bases: A 1H NMR Study.” Journal of the American Chemical Society 116, 4866-4873. |
| 141 | Robinson, C. R., and Sligar, S. G. (1994) “Hydrostatic Pressure Reverses Osmotic Pressure Effects on the Specificity of EcoRI-DNA Interactions.” Biochemistry 33, 3787-3792. PMID: 8142380 |
| 140 | Aikens, J., and Sligar, S. G. (1994) “Kinetic Solvent Isotope Effects During Oxygen Activation by Cytochrome P-450cam. Journal of the American Chemical Society 116, 1143-1144. |
| 139 | Hong, H, Jiang, M., Sligar, S. G., and Bohn, P. W. (1994) “Cysteine-Specific Surface Tethering of Genetically Engineered Cytochromes for Fabrication of Metalloprotein Nanostructures.” Langmuir 10, 153-158. |
| 138 | Feng, Y., Sligar, S. G., and Wand, A. J. (1994) “Solution Structure of Apocytochrome b562.” Nature Structural Biology 1, 30-35. PMID: 7656004 |
| 137 | Lee, K.B., La Mar, G.N., Smith, K.M., Pochapsky, T.C., and Sligar, S.G. (1993) “Interpretation of Hyperfine Shift Patterns in Ferricytochromes b5 in Terms of Angular Position of the Heme: A Sensitive Probe for Peripheral Heme Protein Interactions.” Biochimica et Biophysica Acta 1202, 189-199. PMID: 8399380 |
| 136 | Braunstein, D. P., Chu, K., Egeberg, K. D., Frauenfelder, H., Mourant, J.R., Nienhaus, G. U., Ormos, P., Sligar, S. G., Springer, B. A., and Young, R. D. (1993) “Ligand Binding to Heme Proteins: III. FTIR Studies of His-E7 and Val-E11 Mutants of Carbonmonoxymyoglobin.” Biophysical Journal 65, 2447-2454.
PMID: 8312483 |
| 135 | Nölting, B., and Sligar, S.G. (1993) “Adiabatic Compressibility of Molten Globules.” Biochemistry 32, 12319-12323. PMID: 8241118 |
| 134 | Robinson, C. R., and Sligar, S.G. (1993) “Molecular Recognition Mediated by Bound Water: A Mechanism for Star Activity of the Restriction Endonuclease EcoRI.” J. Mol. Biol. 234, 302-306. PMID: 8230215 |
| 133 | Cortes, R., Ascone, I., Pin, S., and Alpert, B., Chiu, M. L., and Sligar, S. G. (1993) “XAS Study of the Heme Iron Coordination in HIS64(E7)TYR Synthetic Sperm Whale Myoglobin.” Jpn. J. Appl. Phys. 32 Suppl. 2, 544-546. |
| 132 | Loida, P. J., and Sligar, S. G. (1993) “Molecular Recognition in Cytochrome P-450: Mechanism for the Control of Uncoupling Reactions.” Biochemistry 32, 11530-11538. PMID: 8218220 |
| 131 | Nölting, B., Jiang, M., and Sligar, S. G. (1993) “The Acidic Molten Globule State of α-Lactalbumin Probed by Sound Velocity.” Journal of the American Chemical Society 115, 9879-9882. |
| 130 | Koch, T., Ropp, J.D., Sligar, S.G., and Schuster, G.B. (1993) “Photocleavage of DNA: Irradiation of Quinone-Containing Reagents Converts Supercoiled to Linear DNA.” Photochemistry and Photobiology 58, 554-558. PMID: 8248331 |
| 129 | McLean, M., Stayton, P., and Sligar, S. (1993) “Engineering Protein Orientation at Surfaces to Control Macro-Molecular Recognition Events.” Analytical Chemistry 65, 2676-2678. PMID: 8238945 |
| 128 | Ropp, J.D., Gunsalus, I.C., and Sligar, S.G. (1993) “Cloning and Expression of a Member of a New Cytochrome P-450 Family: Cytochrome P-450lin (CYP111) from Pseudomonas incognita.” Journal of Bacteriology 175, 6028-6037. PMID: 8376348 PMCID: PMC206685 |
| 127 | Willie, A., McLean, M., Liu, R., Hilgen-Willis, S., Saunders, A. J., Pielak, G. J., Sligar, S. G., Durham, B., and Millett, F. (1993) “Intracomplex Electron Transfer between Ruthenium-65-Cytochrome b5 and Position-82 Variants of Yeast Iso-1-cytochrome c.” Biochemistry 32, 7519-7525. PMID: 8393343 |
| 126 | Scott, J. R., Willie, A., McLean, M., Stayton, P., Sligar, S., Durham, B., and Millett, F. (1993) “Intramolecular Electron Transfer in Cytochrome b5 Labeled with Trisbipyridylruthenium(II): Rate Measurements in the Inverted Region.” Journal of the American Chemical Society 115, 6820-6824. |
| 125 | Hong, H., Bohn, P. W., and Sligar, S. G. (1993) “Optical Determination of Surface Density in Oriented Metalloprotein Nanostructures.” Analytical Chemistry 65, 1635-1638. |
| 124 | Rajarathnam, K., Qin, J., La Mar,G., Chiu, M., and Sligar, S. G. (1993) “Solution Structure Determination of the Heme Cavity in the E7 His → Val Cyano-met Myoglobin Point Mutant Based on the 1H NMR Detected Dipolar Field of the Iron: Evidence for Contraction of the Heme Pocket.” Biochemistry 32, 5670-5680.
PMID: 8504086 |
| 123 | Robinson, C. R., and Sligar, S. G. (1993) “Electrostatic Stabilization in Four-Helix Bundle Proteins.” Protein Science 2, 826-837. PMID: 8388289 |
| 122 | DiPrimo, C., Hui Bon Hoa, G., Douzou, P., and Sligar, S.G. (1993) “Conformational Dynamics of Cytochrome P-450cam as Monitored by Photo-Acoustic Calorimetry.” Biochemistry 32, 3671-3676.
PMID: 8466907 |
| 121 | Loida, P.J., and Sligar, S.G. (1993) “Engineering Cytochrome P-450cam to Increase the Stereospecificity and Coupling of Aliphatic Hydroxylation.” Protein Engineering 6, 207-212. PMID: 8475046 |
| 120 | Paulsen, M.D., Filipovic, D., Sligar, S.G., and Ornstein, R.L. (1993) “Controlling the Regiospecificity and Coupling of Cytochrome P450cam: T185F Mutant Increases Coupling and Abolishes 3-Hydroxynorcamphor Product.” Protein Science 2, 357-365. PMID: 8453374 PMCID: PMC2142384 |
| 119 | Filipovic, D., Paulsen, M. D., Loida, P. J., Sligar, S. G., and Ornstein, R. L. (1992) “Ethylbenzene Hydroxylation by Cytochrome P450cam.” Biochemistry Biophysics Research Communication, Biochemical and Biophysical Research Communications 189, 488-495. PMID: 1449498 |
| 118 | Davies, M.D., and Sligar, S.G. (1992) “Genetic Variants in Putidaredoxin-Cytochrome P-450cam Electron Transfer Complex: Identification of the Residue Responsible for Redox-State Dependent Stabilization.” Biochemistry 31, 11383-11389. PMID: 1445875 |
| 117 | Cruañes, M.T., Rodgers, K.K., and Sligar, S.G. (1992) “Protein Electrochemistry at High Pressure.” Journal of the American Chemical Society 114, 9660-9661. |
| 116 | Di Primo, C., Hui Bon Hoa, G., Douzou, P., and Sligar, S. G. (1992) “Heme Pocket Hydration Change During the Inactivation of Cytochrome P-450cam by Hydrostatic Pressure.” European Journal of Biochemistry 209, 583-588. PMID: 1425665 |
| 115 | Di Primo, C., Sligar, S.G., Kornblatt, J.A., Hui Bon Hoa, G., and Douzou, P. (1992) “A Critical Role of Protein-Bound Water in the Catalytic Cycle of Cytochrome P-450 Camphor.” FEBS Letters 312, 252-254.
PMID: 1426259 |
| 114 | Stayton, P.S., Jiang, M., Olinger, J.M., Bohn, P.W., and Sligar, S.G. (1992) “Genetic Engineering of Surface Attachment Sites Yields Oriented Protein Monolayers.” Journal of the American Chemical Society 114, 9298-9299. |
| 113 | Rajarathnam, K., La Mar, G., Chiu, M., and Sligar, S. (1992) “Determination of the Orientation of the Magnetic Axes of the Cyano-MetMb Complexes of Point Mutants of Myoglobin by Solution Proton NMR: Influence of HisE7 -> Gly and Arg CD3 -> Gly Substitutions.” Journal of the American Chemical Society 114, 9048-9059. |
| 112 | Van Dyke, B.R., Bakan, D.A., Hegenauer, J.C., Saltman, P., Springer, B.A., and Sligar, S.G. (1992) “Site-directed Mutagenesis of Histidine Residues Involved in Cu(II) Binding and Reduction by Sperm Whale Myoglobin.” Proceedings of the National Academy of Sciences 89, 8016-8019. PMID: 1518828
PMCID: PMC49846 |
| 111 | Gerber, N.C., and Sligar, S.G. (1992) “Catalytic Mechanism of Cytochrome P-450: Evidence for a Distal Charge Relay.” Journal of the American Chemical Society 114, 8742-8743. |
| 110 | Sligar, S.G., and Salemme, F.R. (1992) “Protein Engineering for Molecular Electronics.” Structural Biology 2, 587-592. PMID: 1368440 |
| 109 | Hernan, R.A., Hui, H.L., Andracki, M.E., Noble, R.W., Sligar, S.G., Walder, J.A., and Walder, R.Y. (1992) “Human Hemoglobin Expression in E. coli: Importance of Optimal Codon Usage.” Biochemistry 31, 8619-8628. PMID: 1390646 |
| 108 | Willie, A., Stayton, P., Durham, B., Sligar, S., and Millett, F. (1992) “Genetic Engineering of Redox Donor Sites: Measurement of Intracomplex Electron Transfer Between Ruthenium-65-Cytochrome b5 and Cytochrome c.” Biochemistry 31, 7237-7243. PMID: 1324708 |
| 107 | Bormett, R.W., Asher, S.A., Larkin, P.J., Gustafson, W.G., Ragunathan, N., Freedman, T.B., Nafie, L.A., Balasubramanian, S., Boxer, S.G., Yu, N-T., Gersonde, K., Noble, R.W., Springer, B.A., and Sligar, S.G. (1992) “Selective Examination of Heme Protein Azide Ligand-Distal Globin Interactions by Vibrational Circular Dichroism.” Journal of the American Chemical Society 114, 6864-6867. |
| 106 | Fu, W., Drozdzewski, P.M., Davies, M.D., Sligar, S.G., and Johnson, M.K. (1992) “Resonance Raman and Magnetic Circular Dichroism Studies of Reduced (2Fe-2S) Proteins.” Journal of Biological Chemistry 267, 15502-15510. PMID: 1639790 |
| 105 | Wells, A.V., Li, P., Champion, P.M., Martinis, S.A., and Sligar, S.G. (1992 ) “Resonance Raman Investigations of E. coli-Expressed P. putida Cytochrome P-450 and P-420.” Biochemistry 31, 4384-4394.
PMID: 1581294 |
| 104 | Raag, R., Martinis, S.A., Sligar, S.G., and Poulos, T.L. (1991) “Crystal Structure of the Cytochrome P-450cam Active Site Mutant Thr252Ala.” Biochemistry 30, 11420-11429. PMID: 1742281 |
| 103 | Rodgers, K., and Sligar, S.G. (1991) “Surface Electrostatics, Reduction Potentials, and the Internal Dielectric Constant of Proteins.” Journal of the American Chemical Society 113, 9419-9421. |
| 102 | Wells, A.V, Sage, J.T., Morikis, D., Champion, P., Chiu, M.L., and Sligar, S.G. (1991) “The Iron-Histidine Mode of Myoglobin Revisited: Resonance Raman Studies of Isotopically Labeled E. coli-Expressed Myoglobin.” Journal of the American Chemical Society 113, 9655-9660. |
| 101 | Nikkila, H., Gennis, R., and Sligar, S. (1991) “Cloning and Expression of the Gene Encoding the Soluble Cytochrome b562 of Escherichia coli .” European Journal of Biochemistry 202, 309-313. PMID: 1761034 |
| 100 | Frauenfelder, H., Sligar, S.G., and Wolynes, P.G. (1991) “The Energy Landscapes and Motions of Proteins.” Science 254, 1598-1603. PMID: 1749933 |
| 99 | Rodgers, K.K., and Sligar, S.G. (1991) “Mapping Electrostatic Interactions in Macromolecular Associations.” Journal of Molecular Biology 221, 1453-1460. PMID: 1658337 |
| 98 | Feng, Y., and Sligar, S.G. (1991) “The Effect of Heme Binding to the Structure and Stability of E. coli Apocytochrome b562.” Biochemistry 30, 10150-10155. PMID: 1931945 |
| 97 | Cutruzzola, F., Allocatelli, C., Ascenzi, P., Bolognesi, M., Sligar, S., and Brunori, M. (1991) “Control and Recognition of Anionic Ligands in Myoglobin.” FEBS Letters 282, 281-284. PMID: 2037047 |
| 96 | Rajarathnam, K., La Mar, G.N., Chiu, M., Sligar, S.G., Singh, J.P., and Smith, K. (1991) “1H Hyperfine Shift Pattern as a Probe for Ligation State in High-Spin Ferric Hemoproteins: Water Binding in Metmyoglobn Mutants.” Journal of the American Chemical Society 113, 7886-7892. |
| 95 | Atkins, W.M., and Sligar, S.G. (1991) “Protein Engineering for Studying Enzyme Catalytic Mechanism.” Current Biology 1, 611-616. |
| 94 | Feng, Y., Wand, A.J., and Sligar, S.G. (1991) 1H and 15N NMR Resonance Assignments and Preliminary Structural Characterization of Escherichia Coli Apocytochrome b562.” Biochemistry 30, 7711-7717.
PMID: 1868051 |
| 93 | Carver, T.E., Olson, J.S., Smerdon, S.J., Wilkinson, A.J., Kryzwda, S., Gibson, Q.H., Ropp, J.D., and Sligar, S.G. (1991) “Contributions of Residue 45 (CD3) and Heme-6-propionate to the Bimolecular and Geminate Recombination Reactions of Myoglobin.” Biochemistry 30, 4697 4705. PMID: 2029516 |
| 92 | Park, K., Guo, K., Adebodun, F., Chiu, M., Sligar, S., and Oldfield, E. (1991) “Distal and Proximal Ligand Interactions in Heme Proteins: Correlations between C-O and Fe-C Virbrational Frequencies, Oxygen-17 and Carbon-13 Nuclear Magnetic Resonance chemical Shifts, and Oxygen-17 Nuclear Quadrupole Coupling Constants in C17O- and 13CO-Labeled Species.” Biochemistry 30, 2333-2347. PMID: 2001365 |
| 91 | Qin, L., Rodgers, K., and Sligar, S. (1991) “Electron Transfer Between Cytochrome b5 Surface Mutants and Cytochrome c.” Molecular Crystals and Liquid Crystals 194, 311-316. |
| 90 | Wu, J-Z., La Mar, G., Yu, L., Lee, K-B., Walker, F. Ann, Chiu, M., and Sligar, S. (1991) “1H NMR Study of the Solution Molecular and Electronic Structure of Escherichia coli Ferricytochrome b562: Evidence for S = 1/2 – S = 5/2 Spin Equilibrium for Intact His/Met Ligation.” Biochemistry 30, 2156-2165. PMID: 1998676 |
| 89 | Stayton, P., and Sligar, S.G. (1991) “Structural Microheterogeneity of a Tryptophan Residue Required for Efficient biological Electron Transfer Between Putidaredoxin and Cytochrome P 450.” Biochemistry 30, 1845-1851. PMID: 1993199 |
| 88 | Lee, K., La Mar, G., Kehres, L., Fuginara, E., Smith, K., Pochapsky, P., and Sligar, S. (1990) “1H NMR Study of the Influence of Hydrophobic Contacts on protein-Prosthetic Group Recognition in Bovine and Rat Ferricytochrome b5” Biochemistry 29, 9623-9631. PMID: 2271605 |
| 87 | Di Primo, C., Hui Bon Hoa, G. Douzou, P, and Sligar, S. (1990) “Effect of the Tyrosine-96 Hydrogen Bond on the Inactivation of Cytochrome P-450cam Induced by Hydrostatic Pressure.” European Journal of Biochemistry FEBS 193, 383-386. PMID: 2226459 |
| 86 | Carver, T., Rohlfs, R., Olson, J., Gibson, Q., Blackmore, R., Springer, B., and Sligar, S. (1990) “Analysis of the Kinetic Barriers for Ligand Binding to Sperm Whale Myoglobin Using Site-Directed Mutagenesis and Laser Photolysis Techniques.” The Journal of Biological Chemistry, 265, 20007-20020. |
| 85 | Bellelli, A., Antonini, G., Brunori, M., Springer, B., and Sligar, S. (1990) “Transient Spectroscopy of the Reaction of Cyanide with Ferrous Myoglobin: Effect of Distal Residues.” The Journal of Biological Chemistry 265, 18898-18901. PMID: 2229052 |
| 84 | Davies, M., Qin, L., Beck, J., Suslick, K., Koga, H., Horiuchi, T., and Sligar, S. (1990) “Putidaredoxin Reduction of Cytochrome P-450cam: Dependence of Electron Transfer on the Identity of Putidaredoxin’s C-Terminal Amino Acid.” The Journal of the American Chemical Society 112, 7396-7398. |
| 83 | Kaderbhai, M. A., Sligar, S. G., Barnfield, T., Reames, T., Gallagher, J., He, M., Mercer, E. I., and Kaderbhai, N. (1990) “A Novel Series of pEX-PINK Expression Vectors for Screening High-Level Production of (Un)fused Foreign Proteins by Rapid Visual Detection of PINK Escherichia coli Clones.” Nucleic Acids Research 18, 4629-4630. PMID: 2201954 PMCID: PMC331320 |
| 82 | Hui Bon Hoa, G., Di Primo, C., Geze, M., Douzou, P., Kornblatt, J., and Sligar, S. (1990) “The Formation of P-450 from P-420 is Promoted by Spermine.” Biochemistry 29, 6810-6815. PMID: 2397214 |
| 81 | Stayton, P., and Sligar, S. (1990) “The Cytochrome P-450cam Binding Surface Defined by Site-Directed Mutagenesis and Electrostatic Modeling.” Biochemistry 29, 7381-7386. PMID: 2223769 |
| 80 | Gerber, N., Horiuchi, T., Koga, H., and Sligar, S. (1990) “Identification of 2Fe-2S Cysteine Ligands in Putidaredoxin.” Biochemical and Biophysical Research Communication 169, 1016-1020. PMID: 2163624 |
| 79 | Pochapsky, T., Sligar, S.G., McLachlan, S.J., and La Mar, G.N. (1990) “Relationship Between Heme Binding Site Structure and Heme Orientations of Two Ferrocytochrome b5’s. A Study in Prosthetic Group Recognition.” Journal of American Chemical Society 112, 5258-5263. |
| 78 | Westrick, J.A., Peters, K.S., Ropp, J.D., and Sligar, S.G. (1990) “The Role of the Arginine-45 Salt Bridge in Ligand Dissociaton from Sperm Whale Carboxymyoglobin as Probed by Photoacoustic Calorimetry.” Biochemistry 29, 6741-6746. PMID: 2168740 |
| 77 | Morikis, D., Champion, P., Springer, B., Egeberg, K., and Sligar, S. (1990) “Resonance Raman Studies of Iron-Spin and Axial Coordination in Distal Pocket Mutants of Ferric Myoglobin.” Journal of Biological Chemistry 265, 12143-12145. PMID: 2373683 |
| 76 | Di Primo, C., Hui Bon Hoa, G. Douzou, P, and Sligar, S. (1990) “Mutagenesis of a Single Hydrogen Bond in Cytochrome P-450 Alters Cation Binding and Heme Solvation.” The Journal of Biological Chemistry 265, 5361-5363. PMID: 2318818 |
| 75 | Egeberg, K.D., Springer, B.A., Martinis, S.A., Sligar, S.G., Morikis, D., and Champion, P.M. (1990) “Alteration of Sperm Whale Myoglobin Axial Ligation by Site-Directed Mutagenesis.” Biochemistry 29, 9783-9791. PMID: 2176857 |
| 74 | Egeberg, K.D., Springer, B A., Sligar, S.G., Carver, T., Rohlfs, R.J., and Olson, J.S. (1990) “The Role of the Myoglobin Distal Valine (E11) in Ligand Binding to Sperm Whale Myoglobin.” The Journal of Biological Chemistry 265, 11788-11795. PMID: 2114403 |
| 73 | Rohlfs, R.J., Mathews, A.J., Carver, T.E., Olson, J.S., Springer, B.A., Egeberg, K.D., and Sligar, S.G. (1990) “The Effects of Amino Acid Substitution at Position E7 (Residue 64) on the Kinetics of Ligand Binding to Sperm Whale Myoglobin.” The Journal of Biological Chemistry 265, 3168-3176. PMID: 2303446 |
| 72 | Phillips, G.N. Jr., Arduini, R.M., Springer, B.A., and Sligar, S.G. (1990) “Crystal Structure of Myoglobin from a Synthetic Gene.” Proteins 7, 358-365. |
| 71 | Atkins, W.A., and Sligar, S.G. (1990) “Tyrosine 96 as a Natural Spectroscopic Probe of the Cytochrome P-450cam Active Site.” Biochemistry 29, 1271-1275. |
| 70 | Martinis, S.A., Atkins, W.M., Stayton, P.S., and Sligar, S.G. (1989) “A Conserved Residue of Cytochrome P 450 is Involved in Oxygen Activation.” Journal of the American Chemical Society 111, 9252-9253. |
| 69 | Stayton, P.S., Poulos, T.L., and Sligar, S.G. (1989) “Putidaredoxin Competitively Inhibits Cytochrome P 450cam Associaton: A Proposed Molecular Model.” Biochemistry 28, 8201-8205. |
| 68 | Morikis, D., Springer, B.A., Sligar, S.G., and Champion, P.M. (1989) “Resonance Raman Investigations of Site Directed Mutants of Myoglobin: Effects of Distal Histidine Replacement.” Biochemistry 28, 4791-4800. |
| 67 | Atkins, W.A., and Sligar, S.G. (1989) “Molecular Recognition in Cytochrome P-450: Alteration of Regioselective Alkane Hydroxylation Via Protein Engineering.” Journal of American Chemical Society 111, 2715-2716. |
| 66 | Springer, B.A., Egeberg, K.D., Sligar, S.G., Rohlfs, R.J., Mathews, A.J., and Olson, J.S. (1989) “Discrimination Between Oxygen and Carbon Monoxide and Inhibition of Autooxidation by Myoglobin: Site-Directed Mutagenesis of the Distal Histidine.” The Journal of Biological Chemistry 264, 3057-3060. |
| 65 | Hui Bon Hoa, G., Di Primo, C., Dondaine, I., Sligar, S., Gunsalus, I., and Douzou, P. (1989) “Conformational Changes of Cytochrome P-450cam and P-450lin Induced by High Pressure.” Biochemistry 28, 651-656. |
| 64 | Martinis, S., Sotirious, C., Chang, C., and Sligar, S. (1989) “Characterization of Cytochrome b5 Reconstituted with a Ferric Chlorin.” Biochemistry 28, 879-884. |
| 63 | Olson, J.S., Mathews, A.J., Rohlfs, R.J., Springer, B.A., Egeberg, K.D., Sligar, S.G., Tame, J., Renaud, J.-P., and Nagai, K. (1988) “The Role of the Distal Histidine in Myoglobin and Haemoglobin.” Nature 336, 265-266. |
| 62 | Braunstein, D., Ansari, A., Berendzen, J., Cowen, B.R., Egeberg, K.D., Frauenfelder, H., Hong, M.K., Ormos, P., Sauke, T.B., Scholl, R., Schulte, A., Sligar, S.G., Springer, B.A., Steinbach, P.J., and Young, R.D. (1988) “Ligand Binding to Synthetic Mutant Myoglobin (His E7 \ Gly): Role of the Distal Histidine.” Proceedings of the National Academy of Science USA 85, 8497-8501. |
| 61 | Atkins, W., and Sligar, S. (1988) “The Roles of Active Site Hydrogen Bonding in Cytochrome P-450cam as Revealed by Site-Directed Mutagenesis. The Journal of Biological Chemistry 263, 18842-18849. |
| 60 | Padbury, G., Sligar, S., Labeque, R., and Marnett, L. (1988) “Ferric Bleomycin Catalyzed Reduction of 10 Hydroperoxy 8,12-octadecadienoic Acid. Evidence for Homolytic 0-0 Bond Scission. Biochemistry 27, 7846-7852. |
| 59 | Stayton, P., Fisher, M., and Sligar, S. (1988) “Determination of Cytochrome b5 Association Reactions: Characterization of Metmyoglobin and Cytochrome P-450cam Binding to Genetically Engineered Cytochrome b5.” The Journal of Biological Chemistry 267, 13544-13548. |
| 58 | Rodgers, K., Pochapsky, T., and Sligar, S. (1988) “Probing the Mechanisms of Macro-molecular Recognition: The Cytochrome b5-Cytochrome c Complex.” Science 240, 1657-1659. |
| 57 | Atkins, W.M., and Sligar, S.G. (1988) “Deuterium Isotope Effects in Norcamphor Metabolism by Cytochrome P-450cam: Kinetic Evidence for the Two Electron Reduction of the High-Valent Iron-Oxo Intermeidate.” Biochemistry 27, 1610-1616. |
| 56 | Springer, B.A., and Sligar, S.G. (1987) “High-Level Expression of Sperm Whale Myoglobin in Escherichia coli. Proceedings of the National Academy of Sciences 84, 8961-8965. PMID: 3321062
PMCID: PMC299671 |
| 55 | Bangcharoenpaurpong, O., Martinis, S., Sligar, S., and Champion, P. (1987) “Investigation of the Raman Excitation Profiles of Cytochrome P-450cam.” Journal of Chemical Physics 87, 4273-4284. |
| 54 | Atkins, W., and Sligar, S. (1987) “Metabolic Switching in Cytochrome P-450cam: Deuterium Isotope Effects on Regiospecificity and the Monoxygenase/Oxidase Ratio.” Journal of the American Chemical Society 109, 3754-3760. |
| 53 | Sligar, S., Egeberg, K., Sage, T., Morikis, D., and Champion, P. (1987) “Alteration of Heme Axial Ligands by Site Directed Mutagenesis: A Cytochrome Becomes a Catalytic Demethylase.” Journal of the American Chemical Society 109, 7896-7897. |
| 52 | Jollie, D., Sligar, S., and Schuler, M. (1987) “Purification and Characterization of Microsomal Cytochrome b5 and NADH Cytochrome b5 Reductase from Pisum Sativum.” Plant Physiology 85, 457-462.
PMID: 16665720 PMCID: PMC1054278 |
| 51 | Fisher, M., and Sligar, S. (1987) “Temperature Jump Relaxation Kinetics of the P-450cam Spin Equilibrium.” Biochemistry 26, 4797-4803. PMID: 3663627 |
| 50 | Bangcharoenpaurpong, O., Rizos, A., Champion, P., Jollie, D., and Sligar, S. (1986) “Resonance Raman Detection of Bound Dioxygen in Cytochrome P-450cam.” Journal of Biological Chemistry 261, 8089-8090.
PMID: 3722145 |
| 49 | Beck von Bodman, S., Schuler, M., Jollie, D., and Sligar, S. (1986) “Synthesis, Bacterial Expression, and Mutagenesis of the Gene Coding for Mammalian Cytochrome b5.” Proceedings of the National Academy of Sciences 83, 9443-9447. PMID: 3540940 PMCID: PMC387154 |
| 48 | Fisher, M., White, R., and Sligar, S. (1986) “Pressure Dissociation of a Protein-Protein Electron Transfer Complex.” Journal of the American Chemical Society 108, 6835-6836. |
| 47 | Huang, Y., Hara, T., Sligar, S., Coon, M., and Kimura, T. (1986) “Thermodynamic Properties of Oxidation-Reduction Reactions of Bacterial, Microsomal, and Mitochondrial Cytochrome P 450’s.” Biochemistry 25, 1390-1394. PMID: 3964682 |
| 46 | Unger, B., Gunsalus, I.C., and Sligar, S. (1986) “Nucleotide Sequence of the Pseudomonas putida Cytochrome P-450cam Gene and Its Expression in Escherichia coli.” The Journal of Biological Chemistry 261, 1158-1163. PMID: 3003058 |
| 45 | Backes, W., Jansson, J., Tamburini, P., Gibson, G., Sligar, S., and Schenkman, J. (1985) “Kinetics of Cytochrome P-450 Reduction: Evidence for Faster Reduction of the High Spin Ferric State.” Biochemistry 24, 5130-5136. |
| 44 | Fisher, M., and Sligar, S. (1985) “Control of Heme Protein Redox Potential and Reduction Rate: Linear Free Energy Relation Between Potential and Ferric Spin State Equilibrium. ” Journal of the American Chemical Society 107, 5018-5019. |
| 43 | Fisher, M., and Sligar, S. (1985) “Tyrosine Motions in Relation to the Ferric Spin Equilibrium of Cytochrome P 450cam.” Biochemistry 24, 6696-6701. |
| 42 | Padbury, G., and Sligar, S. (1985) “Chemical Reactivities of Bleomycin.” The Journal of Biological Chemistry 260, 7820-7823. |
| 41 | Fisher, M., Scarlata, S., and Sligar, S. (1985) “High Pressure Investigations of Cytochrome P 450 Spin and Substrate Binding Equilibria.” Archives of Biochemistry and Biophysics 240, 456-463. |
| 40 | Werneke, J., Sligar, S., and Schuler, M. (1985) “Development of Broad Host Range Vectors for Gene Expression and Promoter Screening in Pseudomonas.” Gene 38, 73-84. |
| 39 | Keyes, S., Heimbrook, D., Francasso, P., Rockwell, S., Sligar, S., and Sartorelli, A. (1985) “Chemotherapeutic Attack on Hypoxic Tumor Cells by the Bioreductive Alkylating Agent Mitomycin C.” Advances in Enzyme Regulation 23, 291-307. |
| 38 | Murray, R., and Sligar, S. (1985) “Oxidative Cleavage of 1-Phenyl-1,2 Ethanediol by 4-CN-N,N-Dimethylaniline-N-oxide and Chloro-(5,10,15,20- Tetraphenylporphinato-Chromium (III): A Model for Cholesterol Side Chain Cleavage by Cytochrome P-450scc.” Journal of the American Chemical Society 107, 2186-2187. |
| 37 | White, R., McCarthy, M., Egeberg, K., and Sligar, S. (1984) “Regioselectivity in the Cytochromes P-450: Control by Protein Constraints and by Chemical Reactivities.” Archives of Biochemistry and Biophysics 228, 493-502. |
| 36 | Keyes, S., Fracasso, P., Heimbrook, D., Rockwell, S., Sligar, S., and Sartorelli, A. (1984) “Role of NADPH-Cytochrome C Reductase and DT- Diaphorase in the Biotransformation of Mitomycin C.” Cancer Research 44, 5638-5643. PMID: 6437671 |
| 35 | Heimbrook, D., Murray, R., Egeberg, K., and Sligar, S. (1984) “Demethylation of N,N-Dimethylaniline and p-cyano-N,N-dimethylaniline and their N-oxides by Cytochromes P 450LM2 and P-450cam.” Journal of the American Chemical Society 106, 1514-1515. |
| 34 | Sligar, S., Gelb, M., and Heimbrook, D. (1984) “Bioorganic Chemistry and P-450 Dependent Catalysis.” Xenobiotica 14, 63-86. |
| 33 | Tamburini, P., Gibson, G., Backes, W., Sligar, S., and Schenkman, J. (1984) “Reduction Kinetics of Purified Rat Liver Cytochrome P-450: Evidence for a Sequential Reaction Mechanism Dependent on the Hemoprotein Spin State.” Biochemistry 23, 4526-4533. |
| 32 | Lewis, B.A., and Sligar, S.G. (1983) “Structural Studies of Cytochrome P-450 Using Small Angle X-Ray Scattering.” The Journal of Biological Chemistry 258, 3599-3601. |
| 31 | Gelb, M.H., Toscano, W.A., and Sligar, S.G. (1982) “Chemical Mechanisms of Cytochrome P 450 Oxidation: Spectral and Catalytic Properties of a Manganese Substituted Protein.” Proceedings of the National Academy of Science USA 79, 5758-5762. PMID: 6964386 PMCID: PMC346989 |
| 30 | Backes, W.L., Sligar, S.G., and Schenkman, J.B. (1982) “Kinetics of Hepatic Cytochrome P 450 Reduction: Correlation with Spin State of the Ferric Heme.” Biochemistry 21, 1324 1330. |
| 29 | Kennedy, K., Sligar, S., and Sartorelli, A. (1982) “Activation and Metabolism of Mitomycin C by Liver Microsomes and Nuclei.” Biochemical Pharmacology 31, 2011-2016. |
| 28 | Gelb, M., Malkonen, P., and Sligar, S. G. (1982) “Cytochrome P-450cam Catalyzed Epoxidation of Dehydrocamphor.” Biochemical and Biophysical Research Communications 104, 853-858. |
| 27 | Cole, P.E., and Sligar, S.G. (1981) “Temperature-Jump Measurement of the Spin State Relaxation Rate of Cytochrome P-450cam.” FEBS Letters 133, 252-254. |
| 26 | Schenkman, J.B., Sligar, S.G., and Cinti, D.L. (1981) “Substrate Interaction with Cytochrome P-450.” Pharmacology and Therapeutics 12, 43-71. |
| 25 | Gelb, M., Heimbrook, D., Malkonen, P., and Sligar, S.G. (1982) “Stereochemistry and Deuterium Isotope Effects in Camphor Hydroxylation by Cytochrome P-450cam.” Biochemistry 21, 370-377. (Correction: 21, 3036.) |
| 24 | Gould, P., Gelb, M., and Sligar, S.G. (1981) “Interaction of 5-Bromo camphor with Cytochrome P-450: Production of 5-Ketocamphor from a Mixed Spin State Hemoprotein.” The Journal of Biological Chemistry 256, 6686-6691. |
| 23 | Heimbrook, D., and Sligar, S.G. (1981) “Multiple Mechanisms of Cytochrome P-450 Catalyzed Substrate Hydroxylations.” Biochemical and Biophysical Research Communications 99, 530-535. |
| 22 | White, R., Sligar, S.G., and Coon, M.J. (1980) “Evidence for a Homolytic Mechanism of Peroxide Oxygen-Oxygen Bond Cleavage During Substrate Hydroxylation by Cytochrome P 450.” The Journal of Biological Chemistry 255, 11108-11111. |
| 21 | Backes, W.L., Sligar, S.G., and Schenkman, J.B. (1980) “Cytochrome P-450 Reduction Exhibits Burst Kinetics.” Biochemical and Biophysical Research Communications 97, 860-867. |
| 20 | Sligar, S., Kennedy, K., and Pearson, D. (1980) “Chemical Mechanisms for Cytochrome P-450 Hydroxylation: Evidence for Acylation of Heme Bound Dioxygen.” Proceedings of the National Academy of Sciences USA 77, 1240-1244. PMID: 6929480 PMCID: PMC348467 |
| 19 | Gibson, G.G., Sligar, S.G. Cinti, D.L., and Schenkman, J.B. (1980) “The Effect of Microsomal Fatty Acids and Other Lipids on the Spin State of Partially Purified Cytochrome P-450.” The Journal of Biological Chemistry, 255, 1867-1873. |
| 18 | Henes, J.B., Briggs, M.S., Sligar, S.G., and Fruton, J.S. (1980) “Fluorescence Energy Transfer Studies on the Active Site of Papain.” Proceedings of the National Academy of Sciences 77, 940-943.
PMID: 6928689 PMCID: PMC348398 |
| 17 | Gibson, G., Cinti, D., Sligar, S., and Schenkman, J.B. (1979) “The Effects of Microsomal Lipids on the Spin State of Purified Delipidated Cytochrome P-450.” Biochemical Society Transactions 7, 1289-1291. |
| 16 | Sligar, S. (1979) “A Thermoelectric Cuvette Cooler for Optical Studies in Cryobio-Chemistry.” Cryobiology 16, 506-508. |
| 15 | Sligar, S.G., Cinti, D.L., Gibson, G.G., and Schenkman, J.B. (1979) “Spin State Control of the Hepatic Cytochrome P-450 Redox Potential.” Biochemical and Biophysical Research Communications 90, 925-932. |
| 14 | Cinti, D.L., Sligar, S.G., Gibson, G.G., and Schenkman, J.B. (1979) “Temperature Dependent Spin Equilibrium of Microsomal and Solubilized Cytochrome P-450 from Rat Liver.” Biochemistry 18, 36-42. |
| 13 | Sligar, S.G., and Gunsalus, I.C. (1979) “Proton Coupling in the P-450 Spin and Redox Equilibria.” Biochemistry 18, 2290-2295. |
| 12 | Sligar, S.G. (1976) “Coupling of Spin, Substrate, and Redox Equilibria in Cytochrome P 450.” Biochemistry 15, 5399-5406. PMID: 187215 |
| 11 | Sligar, S.G., and Gunsalus, I.C. (1976) “A Thermodynamic Model of Regulation: Modulation of Redox Equilibria in Camphor Monoxygenase.” Proceedings of the National Academy of Sciences USA 73, 1078-1082. PMID: 1063390 PMCID: PMC430203 |
| 10 | Sligar, S.G., Namtvedt, M.J., Ellis, R.V., and Gunsalus, I.C. (1976) “The Chemistry and Enzymology of Oxygenated Cytochrome P-450.” Hoppe-Seylers Zeitschrift fur Physiologische Chemie 357, 1056-1058. |
| 9 | Hanson, L.K., Eaton, W.A., Sligar, S.G., Gunsalus, I.C., Gouterman, M., and Connell, C.R. (1976) “Origin of the Anomalous Soret Spectra of Carboxy-Cytochrome P-450.” The Journal of the American Chemical Society 98, 2672-2674. PMID: 1262660 |
| 8 | Lipscomb, J.D., Sligar, S.G., Namtvedt, M.J., and Gunsalus, I.C. (1976) “Autooxidation and Hydroxylation Reactions of Cytochrome P-450.” The Journal of Biological Chemistry 251, 1116 1124. PMID: 2601 |
| 7 | Hanson, L.K., Eaton, W.A., and Sligar, S.G. (1975) “Polarized Absorption Spectra of P-450 Single Crystals.” The Pharmacologist 2, 1-7. PMID: 1262660 |
| 6 | Sligar, S. (1975) “A Kinetic and Equilibrium Description of Camphor Hydroxylation by the P 450 Monoxygenase System.” Ph.D. Thesis, University of Illinois, Urbana. |
| 5 | Gunsalus, I.C., Sligar, S., and Debrunner, P. (1975) “Product Formation from Ferrous-Oxy Cytochrome P-450.” Biochemical Society Transactions 3, 82l-826. |
| 4 | Gunsalus, I.C., and Sligar, S. (1976) “Redox Regulation of Cytochrome P-450 Mixed Function Oxidation by Putidaredoxin and Camphor Ligation.” Biochimie. 58, 143-147. PMID: 953050 |
| 3 | Sligar, S., Debrunner, P., Lipscomb, J., Namtvedt, M., and Gunsalus, I.C. (1974) “A Role of the Putidaredoxin COOH-Terminus in P-450 (Cytochrome m) Hydroxylations.” Proceedings of the National Academy of Sciences USA 71, 3906-3910. PMID: 4530269 PMCID: PMC434294 |
| 2 | Sligar, S., Lipscomb, J., Debrunner, P., and Gunsalus, I.C. (1974) “Superoxide Anion Production from the Autooxidation of Cytochrome P-450.” Biochemical and Biophysical Research Communications 61, 290-296. PMID: 4441397 |
| 1 | Sligar, S., and Blum, H. (1971) “Hyperfine Structure of the Hydrogen Atom in CaF2 as a Function of Temperature.” The Physical Review 3B, 3587-3590. |