Description of Research: With the growing demands on agricultural production around the world, it is becoming increasingly important to figure out how to feed the growing population. For thousands of years we have been optimizing our agricultural outputs in livestock species through reproductive selection based on favorable traits, many of which are due to genetic variation. Single nucleotide polymorphisms are responsible for a great deal of either beneficial or undesirable mutations. We are interested in examining how we can use the CRISPR/Cas9 system to induce single-base pair substitutions, thereby improving animal genetics through removal of undesirable or insertion of beneficial mutations.