The first 3/4 Gyr calves were born at Chessie Creek Farm in South Carolina in October 2019. The University of Illinois in collaboration with Chessie Creek Farm produced the 3/4 Gyr calves by in vitro fertilization of oocytes collected by our team from 1/2 blood Holstein-Gyr donors. This generation is the next step in the production of a Girolando cattle herd in the United States. This work is supported by a generous gift from the Ross Foundation.