Services

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DNA Microinjection

CRISPR-mediated gene edited mice are created by injecting/electroporating CRISPR reagents into mouse zygotes. Transgenic founder mice are generated by injecting a transgene into mouse zygotes.  Injected mouse zygotes will be implanted into foster females to carry them to term. Offspring will be transferred to investigator’s protocol for genotyping.

Pricing

  • Campus Price: $6,513
  • Off-Campus Price: $8,590

Note: Additional charges include embryo donor and recipient purchase for each project.

Request DNA Microinjection

  • Schedule an appointment with TMF Director Fuming Pan (fpan@illinois.edu) to discuss the targeting strategy.
  • Have the DNA construct approval from the IBC.
  • Submit a Service Request Form.

Note: Investigators should provide at least 200ug linearized DNA at 0.5ug/ul or higher to the facility.

Gene Targeting

A targeting vector will be introduced into ES cells via electroporation. ES cells will then be grown in appropriate selection media. Around 200 ES cell clones will be picked up, duplicated, and given to the investigator for analysis. Positive ES clones will then be expanded, frozen, and kept in the facility for subsequent blastocyst injection to generate chimeric mice.

Pricing

  • Campus Price: $8,200
  • Off-Campus Price: $10,816

Note: Additional charges include embryo donor and recipient purchase for each project.

Request Gene Targeting

  • Schedule an appointment with TMF Director Fuming Pan (fpan@illinois.edu) to discuss the targeting strategy.
  • Have the DNA construct approval from the IBC.
  • Submit a Service Request Form.

ES Cell Microinjection

Chimeric mice can be generated by microinjection of targeted ES cells into 3.5 days old mouse embryos. The facility will perform the microinjection and transfer injected blastocysts into foster families. Chimeric mice will be transferred to an investigator’s protocol after weaned. Since mouse ES cells are pluripotent and can contribute to all cells in an animal, including gametes, genetic alterations introduced into the ES cells can be propagated in the next generation to form a stable mutant mouse line.

Pricing

  • Campus Price: $6,675
  • Off-Campus Price: $8,804

Note: Additional charges include embryo donor and recipient purchase for each project.

Request ES Cell Microinjection

  • Have their project approval from both IBC and IACUC.
  • Arrange an appointment with TMF Director Fuming Pan (fpan@illinois.edu) to initiate ES cell microinjection.
  • Submit a Service Request Form.

Note: If the investigator already has ES cell clones, please provide the facility with ES cells and specified protocols. The facility will provide at least two chimeric mice per knockout construct.

Cyropreservation

Mouse embryo or sperm can be cryopreserved to prevent accidental loss of a valuable mouse line or to discontinue the maintenance of a mouse colony. About 200 embryos per mouse line will be cryopreserved. Usually two males are required for sperm cryopreservation for each mouse line. In vitro quality control is included for both embryo and sperm freezing.

Pricing

  • Campus Price: $2,238 ($1,119 for Sperm Freezing Without In Vitro Quality Control)
  • Off-Campus Price: Not Applicable

Request Cyropreservation

Note: The investigator will need to have space to store the cryovials once the project is finished.

Rederivation

The Transgenic Mouse Facility will perform experiments to clean a mouse line from pathogen infections. The procedure will include superovulating, mating, and collecting fertilized one-cell embryos. The investigator is responsible the mouse purchase. Females are super-ovulated and bred to males of the line to be rederived. Embryos are collected and pooled, washed to further reduce the risk of pathogen transfer, and then surgically transferred, using aseptic technique, into the reproductive tract of pseudopregnant SPF females. Upon weaning of the pups, DAR will submit surrogate dams for diagnostic testing, including screens for bacterial and viral pathogens.

Pricing

  • Campus Price: $2,275
  • Off-Campus Price: Not Applicable

Note: Additional charges include embryo donor and recipient purchase for each project.

Request Rederivation

  • Schedule an appointment with TMF Director Fuming Pan (fpan@illinois.edu) to discuss the rederivation project.
  • Provide 3 to 4 proven breeder males and 3 to 4 females, both aged 3 to 4 weeks, for each line to be rederived.
  • Submit a Service Request Form.

Note: Investigators are responsible for the per diem from a quarantine room and any related tests.

Mouse Line Recovery

A mouse line can be reconstituted from fresh or frozen embryos through the technique of embryo transfer. It also can be performed with fresh or frozen sperms through in vitro fertilization.

Pricing

  • Campus Price: $2,550
  • Off-Campus Price: Not Applicable

Note: Additional charges include embryo donor and recipient purchase for each project.

Request Mouse Line Recovery

Note: The Investigator is responsible for per diem from the date of the embryo transfer.

Miscellaneous Services

The Transgenic Mouse Facility staff can train or assist individual mouse users in aspects of mouse colony breeding, such as weaning pups, extracting tail DNA, setting up timed mating, dissecting mouse embryos, and handling mouse ES cell cultures. These services are contingent upon the availability of time, equipment, animal health, and other factors.

Pricing

  • Campus Price: $40/hr
  • Off-Campus Price: Not Applicable

Note: Additional charges include embryo donor and recipient purchase for each project

Request a Miscellaneous Service