Publications

Journal articles:

  1. Paul, K*, Sengupta, P*, Ark, ED, Tu, H, Zhao, Y, Boppart SA. (2017) “Coherent control of an opsin in living brain tissue”. Nat. Phys. 13:p1040 (doi:10.1038/nphys4257). [This work describes how current output from retinal based opsins in brain can be controlled using coherent ultrashort pulses.] *Equal contribution
    Nature News and Views highlight; Biophotonics: The Quantum Feeling by E Collini
  2. Raman, R, Cvetkovic, C, Uzel, SGM, Platt, RJ, Sengupta, P, Kamm, RD, Bashir, R. (2016) “Optogenetic skeletal muscle-powered adaptive biological machines”. Proc. Natl. Acad. Sci., 113: p3497. [This work presents light-induced locomotion of 3D printed flexible optogenetic soft robots.]
  3. Koumlis, S, Buecker, D, Moler, G, Eliasson, V, Sengupta, P. (2015) HAMr: A mechanical impactor for investigation of mild TBI induced by repeated concussions. Exp Mechanics 55: p1441. [This work presents design, construction and use of the first generation HAMr device for measuring impact-induced inflammation using in vitro cortical networks.]
  4. Jewett, KA, Taishi, P, Sengupta, P, Roy, S, Davis, CJ, Krueger JM. (2015) “Tumor necrosis factor enhances the sleep-like state and electrical stimulation induces a wake-like state in co-cultures of neurons and glia”. Euro J Neurosci 42: p2078. [This work shows, for the first time, that cultures of neurons and glia can be used to study sleep correlates in a dish.]
  5. Sengupta, P, Roy, S, Krueger, JM. (2011) The ATP-cytokine-adenosine hypothesis: How the brain translates past activity into sleep. Sleep and Biological Rhythms, The Joint Congress of the 6th Congress of Asian Sleep Research and the 34th Annual Meeting of Japanese Society of Sleep Research, 9: p29. [This short review summarizes the ATP-cytokine-adenosine hypothesis and proposes measurement of sleep-correlates from in vitro networks of neuron and glia.]
  6. Sengupta, P, Bosis E, Nachliel E, Gutman M, Smith SO, Mihályné G, Zaitseva I, McLaughlin S. (2009) EGFR juxta-membrane domain, membranes, and calmodulin: kinetics of their interaction. Biophys J 96: p4887. [This article summarizes regulation of EGFR activation via its juxtamembrane domain using FCS and stopped-flow kinetic measurements.]
  7. Wang, J, Sengupta, P, Guo Y, Golebiewska U, Scarlata S. (2009) Evidence for a second, high affinity Gβγ binding site on Gαi(GDP) subunits. J Biol Chem 284: p16906. [This article uses FRET and PCH to show presence of a high affinity binding site on Gαi for Gβγ.]
  8. Sahoo, B, Nag, S, Sengupta, P, Maiti S. (2009) On the stability of the soluble amyloid aggregates. Biophys J. 97: p1454. [This work presents a framework for understanding the stability and growth of toxic soluble amyloid (Aβ1-40 and Aβ1-42) nano-particles, and provides cues for designing effective destabilizing agents.]
  9. Sengupta, P, Philip F, Scarlata S. (2008) Caveolin-1 alters calcium signal duration through specific interaction with the Gαq family of G proteins. J Cell Sci 121: 1363. [This paper presents protein interactions measured at the single molecule level in living cells, and shows that caveolin-1 changes interaction between Gαq and Gβγ subunits.]
  10. Garai K, Sahoo B, Sengupta, P, Maiti S. (2008) Quasi-homogeneous nucleation of amyloid-β yields numerical bounds for the critical radius, the surface tension and the free energy barrier for nucleus formation. J Chem Phys 128: p45102. [This article demonstrates effect of critical radius for amyloid aggregation kinetics.]
  11. Philip F, Sengupta, P, Scarlata S. (2007) Signaling through the bradykinin receptor type 2 and Gαq follows a stoichiometrically limited model. J Biol Chem 282: p19203. [This article shows how bradykinin type-2 receptor is recruited into large membrane domains using single molecule diffusion measurement in living cells. This article was selected as one of the best 100 articles in biology for year 2007.]
  12. Sengupta, P. Ruano MJ, Tebar F, Golebiewska U, Zaitseva I, Enrich C, McLaughlin S, Villalobo A. (2007) The membrane-permeable calmodulin inhibitors (e.g.W-7/W-13) bind to membranes, changing the electrostatic surface potential: dual effect of W-13 on EGFR activation. J Biol Chem 282: p8474. [This paper presents a mechanistic study of EGFR activation.]
  13. Garai K, Sengupta, P, Sahoo B, Maiti S. (2006) Selective destabilization of soluble amyloid beta oligomers by divalent metal ions. Biochem Biophys Res Comm 345: p210. [Article presents effect of metal ions on toxic amyloid oligomers using FCS.]
  14. Digman MA, Sengupta, P, Wiseman P, Brown CM, Horwitz AR, Gratton E. (2005) Fluctuation correlation spectroscopy with a laser scanning microscope: exploiting the hidden time structure. Biophys J 88: pL33. [This article presents how a laser-scanning microscope can be used for diffusion measurement in living cells. The code developed based on this theory is now part of Zeiss LSM 710 analysis routine.]
  15. Digman MA, Brown CM, Sengupta, P, Wiseman P, Horwitz AR, Gratton E. (2005) Measuring fast dynamics in solution and cells with a laser scanning microscope. Biophys J 89: p1317. [This article presents diffusion data from living cells that is collected using a laser-scanning microscope.]
  16. “Ligand-induced monomer/dimer dynamics of the urokinase receptor in live cells by 2P-FLIM/FCS” Gabriele Malengo, Moreno Zamai, Annapaola Andolfo, Parijat Sengupta, Jason DB Sutin, Francesco Blasi, Enrico Gratton, N Sidenius, and Valeria R Caiolfa, Euro Biophys J., 34(6): p536. [This article presents diffusion and life time imaging data of urokinase receptor in living cells that is collected using a laser-scanning microscope and 2-photon excitation.]
  17. Sengupta, P, Garai K, Balaji J, Periasamy N, Maiti S. (2003) Measuring size distribution in highly heterogeneous systems with fluorescence correlation spectroscopy. Biophys J 84: p1977. [This article presents a maximum entropy method (MEM) based algorithm and associated code (MEMFCS) that can be used to analyze FCS data recorded from heterogeneous systems.]
  18. Sengupta, P, Garai K, Shi Y, Callaway DJE, Maiti S. (2003) The amyloid beta peptide, Aβ1-40, is thermodynamically soluble at physiological concentrations. Biochemistry 42: p10506. [Paper on thermodynamic nature of Aβ1-40 in brain showing that possibly the aggregation is triggered by some secondary phenomenon.]
  19. Sengupta, P, Maiti S. (2003) Localized optical probing of biomolecules: fluorescence correlation spectroscopy and multi-photon microscopy. Proc Ind Natl Sci Acad A 69: p1. [Article on simultaneous FCS and MPM measurements from cells.]
  20. Sengupta, P, Balaji J, Maiti S. (2002) Measuring diffusion in cell membranes by fluorescence correlation spectroscopy. Methods 27: p374. [The article describes design of a custom-built FCS spectrometer and how that is used for measurement of protein diffusion in living cell membranes.]
  21. Sengupta, P, Garai K, Maiti S (2002) Aggregation of Alzheimer’s amyloid beta peptide studied by fluorescence correlation spectroscopy. Proceedings of Sixth International Conference on Optoelectronics, Fiber Optics and Photonics, Mumbai, India, 2002: p51. [This paper presents aggregation dynamics of Alzheimer’s amyloid-β peptide.]
  22. Sengupta, P, Balaji, J, Mukherjee, S, Philip, R, Ravindra Kumar G, Maiti S. (2001) Determination of the absolute two-photon absorption cross section of tryptophan. In Multiphoton Microscopy in the Biomedical Sciences, Editors: Ammasi Periasamy, Peter T. C. So., Proceedings of SPIE, 4262: p336 [This paper demonstrates development of theoretical framework for a technique used for determination of absolute value of two-photon absorption cross-section.]
  23. Balaji, J, Sengupta, P, Maiti S. (2001) Probing diffusion and photochemical properties through localized photobleaching. In Multiphoton Microscopy in the Biomedical Sciences, Editors: Ammasi Periasamy, Peter T. C. So., Proceedings of SPIE, 4262: p329 [This paper presents theoretical framework for measurement of molecular diffusion by measuring its rate of photo-bleaching.]
  24. Sengupta, P, Balaji J, Banerjee S, Philip R, Ravindra Kumar G, Maiti, S. (2000) Sensitive measurement of absolute two-photon absorption cross-sections. J Chem Phys 112: p9201. [Paper shows theory and experiment on absolute 2P absorption cross section measurement using a custom-built laser based instrument.]

Invited talks:

  1. “OptoMEA: A Platform for Analyzing Signaling Efficiency of Neuronal Circuits”, Optical Society of America iOptics Seminar, April 22, Urbana, IL, USA (2016)
  2. “RICS, N&B and FLIM” presented as a part of Fluorescence Foundation’s Principles of Fluorescence Techniques Workshop organized by ISS, April 4-7, 2016, Urbana, IL (2016)
  3. “Optogenetics” Neuroscience I and II (Neur598), March 16, Urbana, IL (2016)
  4. “OptoMEA: A Platform for Analyzing Signaling Efficiency of Neuronal Circuits using Multi-Location Extracellular Electrophysiology, Optical Imaging and Optogenetics” presented as a part of the session titled “Molecular, Cellular and Experimental Neuroscience: Reception, Plasticity and New Approaches” in the 60th Annual Meeting of the Biophysical Society, February 26 – March 2, Los Angeles, CA (2016)
  5. “Optogenetic Stimulation of Channelrhodopsin-2 Expressing Neurons Flips Cortical Networks from Low to High Activity State” presented as a part of the session titled “Neurons: Modeling, Synaptic Transmission, and Optogenetics” in the 58th Annual Meeting of the Biophysical Society, February 15-19, San Francisco, CA (2014)
  6. “Activity-Induced Functional State Shift in Model Neuronal Networks” Wyss Institute at Harvard University, March 14, Boston, MA (2012)
  7. “State Oscillations In Mixed Glia/Neuronal Cultures: Gene Expression” presented as a part of symposium titled “Network State Oscillations: The Building Blocks of Sleep” in SLEEP 2011, the 25th Anniversary Meeting of the Associated Professional Sleep Societies, LLC, June 11-15, Minneapolis, MN (2011)

Conference presentations:

  1. “Computational algorithm for assessing inter-neuronal connectivity to optimize optogenetic stimulation and neural circuit activity” Carlos Renteria, Shreya M. Santhanam, Yuan-Zhi Liu, D. Medina, Parijat Sengupta and Stephen A. Boppart, SPIE Photonics West, BIOS, January 27 – February 1, San Francisco, CA, USA (2018)
  2. “Single source for simultaneous tow-photon simulation and imaging based on spectral-temporal modulation of supercontinuum generation” Yuan-Zhi Liu, Carlos Renteria, Sixian You, Yun Sun, Haohua Tu, Parijat Sengupta and Stephen A. Boppart, SPIE Photonics West, BIOS, January 27 – February 1, San Francisco, CA, USA (2018)
  3. “Reconstruction and depixelation of multicore fiber bundle images by fiber bundle rotation” Carlos Renteria, Javier I. Suarez, Parijat Sengupta and Stephen A. Boppart, SPIE Photonics West, BIOS, January 27 – February 1, San Francisco, CA, USA (2018)
  4. “Spectral-temporal modulation of supercontinuum generation for optogenetics” Yuan-Zhi Liu, Haohua Tu, Javier I. Suarez, Parijat Sengupta, and Stephen A. Boppart, SPIE Photonics West, BIOS, January 28 – February 2, San Francisco, CA, USA (2017)
  5. “Spatial control of in vivo optogenetic light stimulation and recording via an imaging fiber bundle” , Javier I. Suarez, Parijat Sengupta, Jon JH Mun, Justin Rhodes and Stephen A. Boppart, SPIE Photonics West, BIOS, January 28 – February 2, San Francisco, CA, USA (2017)
  6. “Tuning mouse embryonic stem cell-derived motor neurons’ activity using optogenetic stimulation” Gelson J. Pagan-Diáz, Caroline Cvetkovic, Rashid Bashir, Parijat Sengupta, Society for Neuroscience Annual Meeting, November 12-16, San Diego, CA, USA (2016)
  7. “Light-evoked responses from channelrhodopsin-2 expressing neuronal cells studied by quantitative phase imaging” Taewoo Kim, Minguang Shan, Viorel Nastasa, Minqi Wang, Parijat Sengupta, Martha U. Gillette and Gabriel Popescu, Society for Neuroscience Annual Meeting, November 12-16, San Diego, CA, USA (2016)
  8. “Effects of a novel α7 nicotinic acetylcholine receptor agonist on neuronal activity evaluated by multi-electrode electrophysiology” Nainika Roy and Parijat Sengupta, Midwest Biomedical Engineering Regional Conference, Biomedical Engineering Society, November 4, Champaign, IL, USA (2016)
  9. “Low-force mechanical impacts modulate calcium dynamics in neurons” Kimberly Sam, Tom Srnak, Parijat Sengupta, Midwest Biomedical Engineering Regional Conference, Biomedical Engineering Society, November 4, Champaign, IL, USA (2016)
  10. 6. “Combinatorial all-optical imaging system for assessing and controlling neural networks” Carlos Renteria, Javier I. Suarez, Parijat Sengupta, Stephen A. Boppart, Midwest Biomedical Engineering Regional Conference, Biomedical Engineering Society, November 4, Champaign, IL, USA (2016)
  11. “Characterization of spontaneous and light-evoked activity of mouse embryonic stem cell derived motor neurons using optogenetic stimulation and multi-electrode electrophysiology” Gelson J. Pagan-Diáz, Caroline Cvetkovic, Rashid Bashir, Parijat Sengupta, Biomedical Engineering Society Annual Meeting, October 5-8, Minneapolis, MN, USA (2016)
  12. “Spatially controlled optogenetic light stimulation and recording platform via imaging fiber bundles” Javier I. Suárez, Parijat Sengupta, Jonathan Guo-Han Mun, Rajashekar Iyer, Justin Rhodes, Martha U. Gillette, Stephen A. Boppart, SPIE BiOS 2016, February 13-14, San Francisco, Ca, USA (2016)
  13. “Modulation of Channelrhodopsin-2 mediated currents by femtosecond pulse shaping” Kush Paul, Eugene D. Ark, Haohua Tu, Youbo Zhao, Yuan-Zhi Liu, Javier I. Suarez, Parijat Sengupta, Stephen A. Boppart, SPIE Photonics West 2016, February 13-18, San Francisco, CA, USA (2016)
  14. “Amyloid-β oligomers alter sensitivity of hippocampal neurons to optogenetic stimulation” Gelson J. Pagandiáz, Minqi Wang, Anjolie Jose, Parijat Sengupta, Neuroscience 2015, The Annual Meeting of Society for Neuroscience, October 17-21, Chicago, IL, USA (2015)
  15. “Temperature modulated stress response of neurons to repetitive low-force mechanical impacts” David C. Kline, Kimberly Sam, Catherine Best-Popescu, Parijat Sengupta, Neuroscience 2015, The Annual Meeting of Society for Neuroscience, October 17-21, Chicago, IL, USA (2015)
  16. “Modulation of Channelrhodopsin-2 mediated currents by complex femtosecond pulse shaping” Kush Paul, Eugene Ark, Haohua Tu, Youbo Zhao, Parijat Sengupta, Stephen A. Boppart, Neuroscience 2015, The Annual Meeting of Society for Neuroscience, October 17-21, Chicago, IL, USA (2015)
  17. “Spatially controlled light stimulation and recording via imaging fiber bundles” Javier Ibarra Suárez, Parijat Sengupta, Jon G-H Mun, Justin Rhodes, Stephen A. Boppart, Neuroscience 2015, The Annual Meeting of Society for Neuroscience, October 17-21, Chicago, IL, USA (2015)
  18. “Quantitative phase imaging (QPI) of optogenetic signals in neurons” Taewoo Kim, Parijat Sengupta, Martha U. Gillette, Gabriel Popescu, Neuroscience 2015, The Annual Meeting of Society for Neuroscience, October 17-21, Chicago, IL, USA (2015)
  19. “Optogenetic Skeletal Muscle Powered 3D Printed Biological Machines” Ritu Raman, Caroline Cvetkovic, Sebastien GM Uzel, Randall J Platt, Parijat Sengupta, Roger D Kamm, Rashid Bashir, Biomedical Engineering Society Annual Meeting, October 7-10, Tampa, FL (2015)
  20. “Spatial and Temporal Analyses of Slow Electrical Oscillations Recorded from In-Vitro Neuronal Networks” Javier Ibarra Suárez, Paritosh Pande, Parijat Sengupta, the 59th Annual Meeting of the Biophysical Society, February 7-11, Baltimore, MD (2015)
  21. “Spatially-resolved in vivo optogenetic stimulation and imaging platform” Stephen A. Boppart, Justin Rhodes, Parijat Sengupta. NSF-NIH BRAIN Initiative Investigators Meeting, November 20-21, Bethesda, MD (2014)
  22. “Development of an in-vitro Experimental Technique for Blunt Impact Brain Trauma,” Stylianos Koumlis, Veronica Eliasson, Parijat Sengupta, ASME 2014 International Mechanical Engineering Congress & Exposition, November 14-20, 2014, Montreal, Canada (2014)
  23. “Novel myosmine derivatives as potential therapeutics for Alzheimer’s disease evaluated in vitro by multi-electrode electrophysiology of a neuronal network and X-ray crystallography” Travis Denton, Todd Talley, Parijat Sengupta, Kwok-Yiu Ho, Janet Bobango, Bridget Mummy, Anh T. Le, Alex Pentecost and Palmer Taylor, Neuroscience 2014, The Annual Meeting of Society for Neuroscience, November 15-19, Washington, DC, USA (2014)
  24. “Prolonged brain derived neurotrophic factor deprivation changes capsaicin and cholecystokinin sensitivity in cultured nodose neurons” Parijat Sengupta, Steven M. Simasko, Neuroscience 2013, The Annual Meeting of the Society for Neuroscience, November 9-13, San Diego, CA (2013)
  25. “Sleep-reminiscent dynamics in isolated neuronal networks: Spatial characteristics” Sandip Roy, James M Krueger, Kathryn Jewett, Parijat Sengupta, Christopher J Davis, Patrick Corrigan, SLEEP 2012, the 26th Annual Meeting of the Associated Professional Sleep Societies, LLC, June 9-13, Boston, MA (2012)
  26. “Altering neuronal firing by changing astrocyte-to-neuron ratio in vitro” Kathryn Jewett, Parijat Sengupta, Christopher J Davis, James M Krueger, SLEEP 2012, the 26th Annual Meeting of the Associated Professional Sleep Societies, LLC, June 9-13, Boston, MA (2012)
  27. “Optogenetic Stimulation Enhances Interleukin-1β Levels in Cultured Neurons” Parijat Sengupta, Kathryn Jewett, James Clinton, James M. Krueger, the 55th Annual Meeting of the Biophysical Society, March 5-9, Baltimore, MD (2011)
  28. “Networks of cultured neurons show oscillatory dynamics” Parijat Sengupta, Christopher J. Davis, James Clinton, Sandip Roy and James M. Krueger, SLEEP 2011, the 25th Anniversary Meeting of the Associated Professional Sleep Societies, LLC, June 11-15, Minneapolis, MN (2011)
  29. “Optogenetic stimulation enhances C-fos and interleukin-1 beta levels in cultured neurons” Kathryn A. Jewett, Parijat Sengupta, Robert Kirkpatrick, James Clinton and James M. Krueger, SLEEP 2011, the 25th Anniversary Meeting of the Associated Professional Sleep Societies, LLC, June 11-15, Minneapolis, MN (2011)
  30. “Caveolin-1 Boosts Clustering of Mu μ- Opioid Receptors in the Plasma Membrane” Parijat Sengupta, Susana Sanchez and Suzanne Scarlata, the 54th Annual Meeting of the Biophysical Society, February 20-24, San Francisco, CA (2010)
  31. “Regulation of G Protein Signals by Membrane Domains” Suzanne Scarlata and Parijat Sengupta FASEB Meeting, FASEB Journal: Meeting Abstract Supplement of 2009, v23: p-83.1 (2009)
  32. “Role of Caveolae in the Duration of G Protein Signals” Parijat Sengupta, Finly Philip and Suzanne Scarlata, the 51st Annual Meeting of the Biophysical Society, March 3-7, Baltimore, MD (2007)
  33. “Membrane-Permeable Calmodulin Inhibitors (e.g. W7) Also Bind Strongly to Membranes, Changing the Electrostatic Surface Potential”, Parijat Sengupta, Urszula Golebiewska, Irina Zaitseva, Francesc Tebar, Carlos Enrich, Antonio Villalobo and Stuart McLaughlin, the 50th Annual Meeting of the Biophysical Society, February 18-22, Salt Lake City, UT (2006)
  34. “Scanning image correlation spectroscopy reveals fast molecular dynamics”, Parijat Sengupta, Michelle Digman, Enrico Gratton, the 49th Annual Meeting of the Biophysical Society, February 12-16, Long Beach, CA (2005)
  35. “Spatio-temporal fluorescence fluctuation analysis of paxillin-EGFP in cellular adhesions using scanning FCS, scanning ICS and PCH”, Michelle Digman, Claire M. Brown, Alan R. Horwitz, Parijat Sengupta, William W. Mantulin and Enrico Gratton, the 49th Annual Meeting of the Biophysical Society, February 12-16, Long Beach, CA (2005)
  36. “The GPI-anchored Urokinase Receptor (uPAR) dynamics on the cells surface followed by fluorescence fluctuation spectroscopy”, Gabriele Malengo, Annapaola Andolfo, Parijat Sengupta, Shih Chu Liao, Beniamino Barbieri, Giuseppe Chirico, Enrico Gratton, Francesco Blasi, Nicolai Sidenius and Valeria R. Caiolfa, the 49th Annual Meeting of the Biophysical Society, February 12-16, Long Beach, CA (2005)
  37. “Ligand-induced monomer/dimer dynamics of the urokinase receptor in live cells by 2P-FLIM/FCS” Gabriele Malengo, Moreno Zamai, Annapaola Andolfo, Parijat Sengupta, Jason DB Sutin, Francesco Blasi, Enrico Gratton, N Sidenius, and Valeria R Caiolfa, Joint 15th IUPAB and 5th EBSA International Biophysics Congress. Montpellier, France, (2005)
  38. “Alzheimer’s in a test tube: A study of amyloid beta peptide aggregation in vitro by fluorescence correlation spectroscopy”, Parijat Sengupta, K. Garai and S. Maiti, the 7th Trombay Symposium on Radiation and Photochemistry, Mumbai, India (2003)
  39. “Soluble oligomers in amyloid beta aggregation: Effect of divalent metal ions”, K. Garai, Parijat Sengupta, David J. E. Callaway, and S. Maiti, the 47th Annual Meeting of the Biophysical Society, March 1-5, San Antonio, TX (2003)
  40. “Aggregation in Biology studied by Fluorescence Correlation Spectroscopy”, Parijat Sengupta, K. Garai, J. Balaji and S. Maiti, International Symposium on Spectroscopy, Structure and Dynamics, Tata Institute of Fundamental Research, Mumbai, India (2002)
  41. “Kinetics of aggregation of amyloid beta peptide”, Parijat Sengupta, K. Garai, David J. E. Callaway, and S. Maiti, the 46th Annual Meeting of the Biophysical Society, February 23-27, San Francisco, CA (2002)
  42. “Aggregation of the amyloid beta peptide by fluorescence correlation spectroscopy”, Parijat Sengupta, K. Garai, David J. E. Callaway, and S. Maiti, APC 2002, the 3rd Asian Photo-chemistry Conference, January 6-11, Mumbai, India (2002)
  43. “Localized photo-excitation: measuring diffusion, multiphoton cross-section, and photobleaching”, J. Balaji, Parijat Sengupta, G. Ravindra Kumar and S. Maiti, the 45th Annual Meeting of the Biophysical Society, February 17-21, Boston, MA (2001)
  44. “Absolute two-photon absorption cross-sections of biologically relevant chromophores measured by far-field Z-scan”, Parijat Sengupta, J. Balaji, R. Phillip, S. Banerjee, G. Ravindra Kumar and S. Maiti, XIII International Biophysical Congress, September 19-24, New Delhi, India(1999)

 

Bioengineering lab group of Parijat Sengupta