• Zhou K, Zhuang S, Liu F, Chen Y, Li Y, Wang S, Li Y, Wen H, Lin X, Wang J, Huang Y, He C, Xu N, Li Z, Xu L, Zhang Z, Chen LF*, Chen R*, Liu M*. 2022. Disrupting the Cdk9/Cyclin T1 heterodimer of 7SK snRNP for the Brd4 and AFF1/4 guided reconstitution of active P-TEFb. Nuclear Acid Res. 50:750-762. [PubMed] (*corresponding author).


  • Dong XC and Chen LF, 2021. Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages. STAR Protocols. [Abstract]
  • Hu XM, Dong XC, Li G, Chen J, He X, Sun H, Kim DH, Kemper JK, Chen LF. 2021. Brd4 modulates diet-induced obesity via PPARγ-dependent Gdf3 expression in adipose tissue macrophages. JCI Insight .6:e143379. [PubMed]
  • Dong XC, Hu XM, Bao Y, Li G, Yang XD, Slauch JM and Chen LF, 2021. Brd4 regulates NLRC4 inflammasome activation by facilitating IRF8-mediated transcription of Naips. JCB. 220:e202005148. [PubMed]


  • Jung H, Chen J, Hu X, Sun H, Wu S, Chiang C, Kemper B, Chen LF * and Kemper, JK*. 2020. BRD4 inhibition and FXR activation, individually beneficial in cholestasis, are antagonistic in combination. JCI Insight 6:e141640. [PubMed] (*corresponding author)
  • Chen YH, Sheppard D, Dong XC, Hu XM, Chen MH, Chen RC, Chakrabarti J, Zavros Y, Peek RM, and Chen LF. 2020. H. pylori infection confers resistance to apoptosis via Brd4-dependent BIRC3 eRNA synthesis. Cell Death & Disease 11:667. [PubMed]
  • Hu X, Chen LF. 2020. Pinning Down the Transcription: A Role for Peptidyl-Prolyl cis-trans Isomerase Pin1 in Gene Expression. Front Cell Dev Biol. 8:179. [PubMed]
  • Yang XD, Li WG, Zhang S, Wu D, Jiang XL, Tan R, Niu XY, Wang Q, Wu XF, Liu ZD, Chen LF, Qin J, Su B. 2019. PLK4 deubiquitination by Spata2-CYLD suppresses NEK7-mediated NLRP3 inflammasome activation at the centrosome. EMBO J. 39:e102201. [PubMed]
  • Dong XC, Hu XM, Chen J, Hu D and Chen LF. 2018. BRD4 regulates cellular senescence in gastric cancer cells via E2F/miR-106b/p21 axis. Cell Death & Disease 9:203. [PubMed]
  • Zhao XL, Chen JJ, Si SY, Chen LF, Wang Z. 2018. T63 inhibits osteoclast differentiation through regulating MAPKs and Akt signaling pathways. Eur J Pharmacol. 834:30-35. [PubMed]
  • Li Y, Liu M, Chen LF § and Chen R§. 2018. P-TEFb: finding its ways to release promoter-proximally paused RNA Polymerase II. Transcription. 9:88-94. [PubMed] (§corresponding author)


  • Xiong MH, Bao Y,, Xu X, Wang H, Han Z, Wang Z, Liu YY, Huang SY, Song ZY, Chen J, Peek RM, Yin LC,Chen LF*, and Cheng JJ*. 2017. Selective killing of Helicobacter pylori with pH-responsive helix–coil conformation transitionable antimicrobial polypeptides Proc. Natl. Acad. Sci. U.S.A. 114:12675-12680. [PubMed] (*corresponding author)
  • Zho X, Chen J, Zhang GN, Wang Y, Si SY, Chen LF*, Wang Z*. 2017. Small molecule T63 suppresses osteoporosis by modulating osteoblast differentiation via BMP and WNT signaling pathways. Scientific Reports.7:10397.(PubMed)(*corresponding author)
  • Hu XM, Dong SH, Chen J, Zhou XZ, Chen R, Lu KP, Chen LF. 2017. Prolyl isomerase Pin1 regulates the stability, transcriptional activity and oncogenic potential of Brd4. Oncogene. 36:5177-5188 (PubMed)
  • Bao Y, Wu XW, Chen JJ, Hu XM, Zeng FX, Cheng JJ, Jin H, Lin X and Chen LF. 2017. Brd4 modulates the innate immune response through Mnk2-eIF4E pathway-dependent translational control of IκBα. Proc. Natl. Acad. Sci. U.S.A. 114(20):E3993-E4001. (PubMed)
  • Hu D, Peng F, Lin XD, Chen G, Zhang HJ, Liang BY, Ji KD, Lin JX, Chen LF*, Zheng XW*, Niu WQ*. 2017. 2017. Preoperative Metabolic Syndrome Is Predictive of Significant Gastric Cancer Mortality after Gastrectomy: The Fujian Prospective Investigation of Cancer (FIESTA) Study. EBioMedicine 15: 73–80 (*corresponding author) (PubMed)
  • Li Y, Liu M, Chen LF* and Chen R*. 2017. P-TEFb: finding its ways to release promoter-proximally paused RNA Polymerase II. Transcription. doi: 10.1080/21541264.2017.1281864. [Epub ahead of print] (*corresponding author) (PubMed)
  • Zhou JJ, Qu ZX, Sun F, Han L, Yan SP, Stabile LP, Chen LF, Siegfried JM, and Xiao G. 2017. Myeloid STAT3 promotes lung tumorigenesis through transforming tumor immunosurveillance into tumor-promoting inflammation. Cancer Immunology Research 5:257-268 (PubMed)


  • Lu X, Zhu X, Li Y, Liu M, Yu B, Wang Y, Rao M, Yang H, Zhou K, Wang Y, Chen Y, Chen M, Zhuang S, Chen LF*, Liu R*, Chen R*. 2016. Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II. Nucleic Acids Res. 2016 Jun 28. pii: gkw571. [Epub ahead of print] (PubMed) (*corresponding author)
  • Chen JJ, Wang Z, Hu, XM, Chen R, Romero-Gallo J, Perk RM. and Chen LF. 2016. BET inhibition Attenuates H. pylori-induced Inflammatory Response by Suppressing Inflammatory Gene Transcription and Enhancer Activation. J. of Immunology 196:4132-42. (PubMed)


  • Chen JJ and Chen LF. 2015. Methods to detect NF-κB acetylation and methylation. Methods in Molecular Biology. 1280:395-409 (PubMed)
  • Xiong M, Lee MW, Mansbach RA, Song Z, Bao Y, Peek RM Jr, Yao C, Chen LF, Ferguson AL, Wong GC, Cheng J. 2015. Helical antimicrobial polypeptides with radial amphiphilicity. Proc Natl Acad Sci U S A. 112:13155-60. (PubMed)


  • Kim DH, Xiao Z, Kwon S, Sun X, Tkac D, Ryerson D, Choi SE, Ma P, Wi S, Chiang CM, Palvimo J, Chen LF, Kemper B, and J. K. Kemper. 2014. A dysregulated Acetyl/SUMO switch of FXR promotes hepatic inflammation in obesity. EMBO Journal 34:184-99. (PubMed)
  • Zou ZH, Huang B, Wu XW, Zhang HJ, Qi J, Bradner J, Nair S and Chen LF. 2014. Brd4 maintains constitutively active NF-κB in cancer cells by binding to acetylated RelA. Oncogene. 33:2395-404. (PubMed)


  • Wu XW, Qi J, Bradner JE, Xiao GT and Chen LF. 2013. Bromodomain and extra-terminal (BET) protein inhibition suppresses HTLV-1 Tax-mediated tumorigenesis by inhibiting NF-κB signaling. J. Biol. Chem 288:36094-105. (PubMed)
  • Lamb A, Chen JJ, Blanke SR and Chen LF. 2013. Helicobacter pylori activates NF-κB by inducing Ubc13-mediated ubiquitination of lysine 158 of TAK1. J. of Cell Biochem. 114:2284-92. (PubMed)
  • Masuda-Ozawa T, Hoang T, Seo YS, Chen LF, Spies M. 2013. Single-molecule sorting reveals how ubiquitylation affects substrate recognition and activities of FBH1 helicase. Nucleic Acids Res. 41:3576-3587. (PubMed)


  • Lamb A and Chen LF. 2012. Role of the Helicobacter pylori-induced inflammatory response in the development of gastric cancer. J. of Cell Biochem. 114:491-7 (PubMed)
  • Tsang YH, Wu XW, Lim JS, Chee WO, Salto-Tellez M, Ito K, Ito Y, Chen LF. 2012. Prolyl isomerase Pin1 down-regulates tumor suppressor RUNX3 in breast cancer. Oncogene. 32:1488-96 (PubMed)
  • Lim JH, Jono H, Komatsu K, Woo CH,Lee JY, Miyata M, Matsuno T, Xu XB, Huang YX, Zhang WH, Park SH, Kim YI, Choi YD, Shen HH, Heo KS, Xu HD, Bourne P, Koga T, Xu HD, Yan C, Wang BH, Chen LF, Feng XH and Li JD. 2012. CYLD negatively regulates transforming growth facbor-β signaling via deubiquitinating Akt. Nature Communications. 3:771 (PubMed)
  • Chen LF. 2012. Tumor suppressor function of RUNX3 in breast cancer. J. of Cell Biochem 113:1470-1477 (PubMed)


  • Huang B, Qu ZX, Chee WO, Tsang YH, Xiao GT, Shapiro D, Salto-Tellez M, Ito K, Ito Y, Chen LF. 2011. RUNX3 acts as a tumor suppressor in breast cancer by targeting estrogen receptor α. Oncogene. 31:527-34 (PubMed)
  • Yang XD and Chen LF. 2011.Talking to histone: methylated RelA serves as a messenger. Cell Res. 21:561-563 (PubMed)
  • Tsang YH, Lamb A and Chen LF. 2011. New insights into the inactivation of gastric tumor suppressor RUNX3: the role of Helicobacter pylori infection. J. of Cell Biochem. 112:381–386 (PubMed)


  • Tsang YH, Lamb A, Romero-Gallo J, Huang B, Ito K, Peek RM, Ito Y and Chen LF. 2010. Helicobacter pylori CagA targets gastric tumor suppressor RUNX3 for proteasome-mediated degradation. Oncogene. 29:5643-50 (PubMed) (Featured article)
  • Huang B, Yang XD, Lamb A and Chen LF. 2010. Posttranslational modifications of NF-κB: another layer of regulation for NF-κB signaling pathway. Cell Signal 22:1282-90 (PubMed)
  • Lamb A and Chen LF. 2010. The many roads traveled by Helicobacter pylori to NF-κB activation. Gut Microbes. 1: 109-113 (PubMed)
  • Yang XD, Tajkhorshid E., Chen LF. 2010. Functional interplay between acetylation and methylation of the RelA subunit of NF-κB. Mol. Cell. Biol. 30:2170-2180 (PubMed)


  • Nossa CW, Jain P, Batcha T, Gupta VR, Chen LF, Schreiber V, Desnoyers S, Blanke SR. 2009. Activation of the abundant nuclear factor poly(ADP- 2 ribose) polymerase-1 by Helicobacter pylori. Proc. Natl. Acad. Sci. USA. 106:19998-20003 (PubMed)
  • Yang XD, Lamb A, Chen LF. 2009. Methylation, a new epigenetic mark for protein stability. Epigenetics. 4:429-33 (PubMed)
  • Lamb A, Yang XD, Tsang YH, Li JD, Higashi H, Hatakeyama M, Peek RM, Blanke SR, Chen LF. 2009. Helicobacter pylori CagA activates NF-κB by inducing TRAF6-mediated K63-ubiquitination of TAK1. EMBO Reports. 10:1242-1249 (PubMed)
  • Yang XD, Huang B, Li MX, Lamb A, Kelleher NL, Chen LF. 2009. Negative regulation of NF-κB action by Set9-mediated lysine methylation of RelA subunit. EMBO J. 28:1055-1066 (Pubmed)
  • Huang B, Yang XD, Zhou MM, Ozato K, Chen LF. 2009. Brd4 coactivates transcriptional activation of NF-κB via specific binding to acetylated RelA. Mol. Cell. Biol. 29:1375-87 (PubMed)
  • Ishinaga H., Jono H., Lim JH, Komatsu K, Xu X, Lee J, Woo CH, Xu H, Feng XH, Chen LF, Yan C, Li JD. 2009. Synergistic induction of NF-κB by transforming growth factor β and tumour necrosis factor-α is mediated by protein kinase A-dependent RelA acetylation. Biochem. J. 417:583-91 (PubMed)


  • Kwon H., Brent MM, Getachew R, Jayakumar P, Chen LF, Schnoelzer M., McBurney MW, Marmorstein R, Greene WC, and Ott M. 2008. Human immunodeficiency virus type 1 Tat protein inhibits the SIRT1 deacetylase and induces T-cell hyperactivation. Cell Host Microbe. 3:158-67 (PubMed)
  • Murakami Y, Chen LF, Sanechika N, Kohzaki H, Ito Y. 2007. Transcription factor Runx1 recruits the polyomavirus replication origin to replication factories. J. Cell Biochem. 100(5):1313-23 (PubMed)
  • Williams S, Kwon H, Chen LF, Greene WC. 2007. Sustained Induction of NF-κB Is required for efficient expression of latent HIV-1. J Virol. 81:6043-56 (PubMed)
  • Ishinaga H, Jono H, Lim JH, Kweon SM, Xu H, Ha UH, Xu H, Koga T, Yan C, Feng XH, Chen LF*, Li JD*. 2007. TGFβ induces p65 acetylation to enhance bacteria-induced NF-κB activation. EMBO J. 26:1150-1162 (*corresponding author) (PubMed)
  • Williams S, Chen LF, Kwon H, Ruiz-Jarabo CM, Greene WC. 2006. NF-κB p50 promotes HIV latency through HDAC recruitment and repression of transcriptional initiation. EMBO J.25:139-149 (PubMed)


  • Chen J, Zhou Y, Mueller-Steiner S, Chen LF, Kwon H, Yi S, Mucke L, Gan L. 2005. SIRT1 Protects against Microglia-dependent Amyloid-b Toxicity through Inhibiting NF-κB Signaling. J. Biol. Chem.280: 40364-40374 (PubMed)
  • Chen LF*, Williams S, Mu Y, Nakano H, Duerr JM, Buckbinder L, and Greene WC*. 2005. RelA phosphorylation regulates RelA acetylation. Mol. Cell. Biol. 25:7966-7975 (*corresponding author) (PubMed)
  • Chen LF, Greene WC. 2005. Assessing acetylation of NF-κB. Methods.36:368-375 (Pubmed)
  • Williams S., Chen LF, Kwon H, Fenard D, Bisgrove1 D, Verdin E, Greene WC. 2004. Prostratin antagonizes HIV latency by activating NF-κB. J. Biol. Chem. 279: 42008-42017 (PubMed)
  • Jono H, Lim JH, Chen LF, Xu H, Trompouki E, Pan ZK, Mosialos G, Li JD. 2004. NF-κB is essential for induction of CYLD, the negative regulator of NF-κB: Evidence for a novel inducible auto-regulatory feedback pathway. J. Biol. Chem.279:36171– 36174 (PubMed)
  • Chen LF, Greene WC. 2004. Shaping the nuclear action of NF-κB. Nat. Rev. Mol. Cell. Biol. 5:392–401 (PubMed)
  • Bohuslav J, Chen LF, Kwon H, Mu Y, Greene WC. 2004. p53 induces NF-κB activation by an IκB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1 (RSK1). J. Biol. Chem. 279:26115–26125 (PubMed)
  • O’Mahony A, Montano M, Van Beneden K, Chen LF, Greene WC. 2004. HTLV-I Tax induction of biologically active NF-κB requires IKK1-mediated phosphorylation of RelA/p65. J. Biol. Chem. 279:18137–18145 (PubMed)
  • Chen LF, Greene WC. 2003. Regulation of distinct biological activities of the NF-κB transcription factor complex by acetylation. J. Mol. Med. 81:549–557 (PubMed)
  • Guo WH, Weng LQ, Ito K, Chen LF, Nakanishi H, Tatematsu M, Ito Y. 2002. Inhibition of growth of mouse gastric cancer cells by Runx3, a novel tumor suppressor. Oncogene. 28:8351-5 (PubMed)
  • Chen LF, Mu Y, Greene WC. 2002. Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-κB. EMBO J. 21: 6539–6548 (PubMed)
  • Chen LF, Fischle W, Verdin E, Greene WC. 2001. Duration of nuclear NF-κB action regulated by reversible acetylation. Science 293:1653–1657 (PubMed)
  • Foehr ED, Bohuslav J, Chen LF, DeNoronha C, Geleziunas R, Lin X, O’Mahony A, Greene WC. 2000. The NF-κB-inducing kinase induces PC12 cell differentiation and prevents apoptosis. J. Biol. Chem. 275: 34021–34024 (PubMed)


  • Hanai J,* Chen LF,* Kanno T, Ohtani-Fujita N, Kim WY, Guo WH, Imamura T, Ishidou Y, Fukuchi M, Shi MJ, Stavnezer J, Kawabata M, Miyazono K, Ito Y. 1999. Interaction and functional cooperation of PEBP2/CBF with Smads. Synergistic induction of the immunoglobulin germline Ca promoter. J. Biol. Chem.274:31577–31582 (*equal contribution) (PubMed)
  • Yagi R, Chen LF, Shigesada K, Murakami Y, Ito Y. 1999. A WW domain-containing yes-associated protein (YAP) is a novel transcriptional co-activator. EMBO J.18:2551–2562 (PubMed)
  • Chen LF, Ito K, Murakami Y, Ito Y. 1998.The capacity of polyomavirus enhancer binding protein 2αB1(AML1/Cbfα2) to stimulate polyomavirus DNA replication is related to its affinity for the nuclear matrix. Mol. Cell. Biol.18: 4165–4176 (PubMed)
  • Kanno T, Kanno Y, Chen LF, Ogawa E, Kim WY, Ito Y. 1998. Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/Core binding factor α subunit revealed in the presence of the β subunit. Mol. Cell. Biol. 18:2444–2454 (PubMed)

U.S. Patents

1. US Patent 7,081,343 – Methods for identifying modulators of NF-B activity, Issued on July 25, 2006. Inventors: Lin-Feng Chen, Wolfgang Fishcle, Eric Verdin, Warner C. Greene.
2. US Patent 11,225,507 – Conformation switchable antimicrobial peptides and methods of using the same. Issued on January 18, 2022. Inventors: Jianjun Cheng, Lin-Feng Chen, Menghua Xiong, Yan Bao