Mechanisms of Oxidative Damage

  1. Imlay, J. A. The mismetallation of enzymes during oxidative stress. J. Biol. Chem. 289, 28121–28128 (2014).
  2. Sobota, J. M., Gu, M. & Imlay, J. A. Intracellular hydrogen peroxide and superoxide poison 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, the first committed enzyme in the aromatic biosynthetic pathway of Escherichia coli. J. Bacteriol. 196, 1980–1991 (2014).
  3. Imlay, J. A. The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium. Nat. Rev. Microbiol. 11, 443–54 (2013).
  4. Gu, M. & Imlay, J. A. Superoxide poisons mononuclear iron enzymes by causing mismetallation. Mol. Microbiol. 89, 123–134 (2013).
  5. Anjem, A. & Imlay, J. A. Mononuclear iron enzymes are primary targets of hydrogen peroxide stress. J. Biol. Chem. 287, 15544–15556 (2012).
  6. Sobota, J. M. & Imlay, J. a. Iron enzyme ribulose-5-phosphate 3-epimerase in Escherichia coli is rapidly damaged by hydrogen peroxide but can be protected by manganese. Proc. Natl. Acad. Sci. U. S. A. 108, 5402–5407 (2011).
  7. Jang, S. & Imlay, J. A. Hydrogen peroxide inactivates the Escherichia coli Isc iron-sulphur assembly system, and OxyR induces the Suf system to compensate. Mol. Microbiol. 78, 1448–1467 (2010).
  8. Jang, S. & Imlay, J. a. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. J. Biol. Chem. 282, 929–37 (2007).
  9. Macomber, L., Rensing, C. & Imlay, J. A. Intracellular copper does not catalyze the formation of oxidative DNA damage in Escherichia coli. J. Bacteriol. 189, 1616–1626 (2007).
  10. Varghese, S., Wu, A., Park, S., Imlay, K. R. & Imlay, J. A. Submicromolar hydrogen peroxide disrupts the ability of fur protein to control free-iron levels in Escherichia coli. Mol. Microbiol. 64, 822–830 (2007).
  11. Park, S., You, X. & Imlay, J. a. Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 102, 9317–22 (2005).
  12. Imlay, J. A. & Fridovich, I. (1991) An antioxidant role for hemoglobin. In Davies, K.J.A. (Ed. ) Oxidative Damage and Repair: Chemical, Biological, and Medical Aspects (pp. 87-92) Oxford: Pergamon Press.
  13. Imlay, J. A. Pathways of oxidative damage. Annu. Rev. Microbiol. 57, 395–418 (2003).
  14. Park, S. & Imlay, J. a. High levels of intracellular cysteine cromote oxidative DNA damage by driving the Fenton reaction. J. Bacteriol. 185, 1942–1950 (2003).
  15. Varghese, S., Tang, Y. & Imlay, J. A. Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletion. J. Bacteriol. 185, 221–230 (2003).
  16. Woodmansee, A. N. & Imlay, J. A. A mechanism by which nitric oxide accelerates the rate of oxidative DNA damage in Escherichia coli. Mol. Microbiol. 49, 11–22 (2003).
  17. Imlay, J. A. How oxygen damages microbes: oxygen tolerance and obligate anaerobiosis. Adv. Microb. Physiol. 46, 111–153 (2002).
  18. Woodmansee, A. N. & Imlay, J. A. Reduced flavins promote oxidative DNA damage in non-respiring Escherichia coli by delivering electrons to intracellular free iron. J. Biol. Chem. 277, 34055–34066 (2002).
  19. Pan, N. & Imlay, J. A. How does oxygen inhibit central metabolism in the obligate anaerobe Bacteroides thetaiotaomicron? Mol. Microbiol. 39, 1562–1571 (2001).
  20. Srinivasan, C., Liba, A., Imlay, J. A., Valentine, J. S. & Gralla, E. B. Yeast lacking superoxide dismutase(s) show elevated levels of ‘free iron’ as measured by whole cell electron paramagnetic resonance. J. Biol. Chem. 275, 29187–29192 (2000).
  21. Maringanti, S. & Imlay, J. A. An intracellular iron chelator pleiotropically suppresses enzymatic and growth defects of superoxide dismutase-deficient Escherichia coli. J. Bacteriol. 181, 3792–3802 (1999).
  22. Keyer, K. & Imlay, J. A. Superoxide accelerates DNA damage by elevating free-iron levels. Proc. Natl. Acad. Sci. U. S. A. 93, 13635–13640 (1996).
  23. Imlay, J. A., Chin, S. M. & Linn, S. Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro. Science 240, 640–2 (1988).
  24. Imlay, J. a & Linn, S. Damage and Oxygen Radical. Science (80-. ). 240, 1302–1309 (1988).
  25. Imlay, J. A. & Linn, S. Bimodal pattern of killing of DNA-repair-defective or anoxically grown Escherichia coli by hydrogen peroxide. J. Bacteriol. 166, 519–527 (1986).