While policy details vary slightly among journals, there is a consensus that the final image must remain consistent with the original data. Unacceptable manipulations generally include adding to, altering, moving or removing a specific feature of an image. Acceptable manipulations generally include adjustments of contrast, brightness, or color that must be applied to an entire image equally. Often, where figures are assembled from multiple images or non-concurrent portions of the same image, these separate parts must be indicated. In many cases, authors will be asked to list all the tools used for image acquisition and processing, and to document key image-gathering settings and manipulation processes if any. Overall, the general rule of thumb is that the final image for publication must correctly represent the original data and conform to community standards.
For an example of illegitimate digital image manipulation, see Mike Rossner’s analysis of a paper published on stem cell research that appeared in Science in 2005.
The following resources about digital image manipulation and scientific publishing provide further information.
- American Chemical Society publications, http://pubs.acs.org/userimages/ContentEditor/1218054468605/ethics.pdf
- Biology Open, http://bio.biologists.org/site/journal/editorial_policies.xhtml#text
- BJOG: An International Journal of Obstetrics and Gynaecology, http://www.bjog.org/view/0/editorialPolicy.html
- Diabetes Journal, http://diabetes.diabetesjournals.org/content/61/1/38.full
- Gastroenterology, http://www.gastrojournal.org/authorinfo#image
- Journal of Cell Science, http://jcs.biologists.org/site/journal/editorial_policies.xhtml#text
- Journal of Experimental Biology, http://jeb.biologists.org/site/journal/editorial_policies.xhtml#image
- Journal of Physiology, http://journals.physoc.org/site/misc/policy.xhtml#manipulation
- Nature journals, http://www.nature.com/authors/policies/image.html
- Nature Methods, http://www.nature.com/nmeth/about/ed_policies/index.html#digital
- New England Journal of Medicine, http://www.nejm.org/page/author-center/technical-guidelines
- Proceedings of the Naitonal Academy of Sciences of the USA, http://www.pnas.org/site/authors/journal.xhtml
- Science, http://www.sciencemag.org/site/feature/contribinfo/prep/prep_revfigs.xhtml
- Bosch, X., et al. (2012). “Misconduct Policies in High-Impact Biomedical Journals”. PLoS ONE 7(12): e51928. doi:10.1371/journal.pone.0051928, http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0051928
- Cromey, D. (2013). “Digital Images Are Data: And Should Be Treated as Such”. In Taatjes & Roth (Eds.), Cell Imaging Techniques (pp. 1-27). Humana Press, http://link.springer.com/protocol/10.1007%2F978-1-62703-056-4_1
- Cromey, D. (2010). “Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images”. Science and Engineering Ethics 16:639–667, http://scienceimageintegrity.org/wp-content/uploads/2010/05/TwistedPixils-Cromey-SEE-Dec2010.pdf
- Cromey, D. (2009). “Digital Image Ethics”. The University of Arizona, http://swehsc.pharmacy.arizona.edu/micro/digital-image-ethics
- Rossner, M. (2007). “Hwang case review committee misses the mark.” Journal of Cell Biology 176(2): 131-132, http://jcb.rupress.org/content/176/2/131.full.pdf
- Rossner, M. and Yamada, K. (2004). “What’s in a picture? The temptation of image manipulation”. Journal of Cell Biology 166(1):11-15. The Rockefeller University Press, doi: 10.1083/jcb.200406019, http://jcb.rupress.org/content/166/1/11.full
- Science Image Integrity, http://scienceimageintegrity.org/