The traditional methods for measuring the oxidative potential of ambient particles have been very laborious and time-consuming. These constraints have always limited the wide-spread use of this important property of the particles in large-scale epidemiological and toxicological studies. Our group has developed the first field-deployable online instrument for measuring the real-time oxidative potential of ambient aerosols based on the dithiothreitol assay. Recently, we have developed a fully automated instrument which measures the oxidative potential of the ambient particles based on all known endpoints, i.e. ascorbate consumption, glutathione consumption, dithiothreitol consumption, hydroxyl radical generation and macrophage ROS generation (patent pending on these instruments). The instruments developed by our group have been used in many field campaigns throughout the world (India, USA, China) by several research groups. These instruments provide highly time-resolved data on PM oxidative stress, which are useful in the downstream exposure assessment and source apportionment studies.
A Realtime Instrument for measuring the Oxidative Potential of Ambient PM2.5 based on the DTT assay and its Field Deployment
A Semi-Automated Multi-Endpoint ROS Activity Analyzer (SAMERA) developed in our lab
A Semi-Automated Instrument for Cellular Oxidative Potential Evaluation (SCOPE) developed in our lab
Related Publications
Puthussery, J.V., Zhang, C., Verma, V., 2018. Development and field testing of an online instrument for measuring the real-time oxidative potential of ambient particulate matter based on dithiothreitol assay, Atmospheric Measurement Techniques, 11, 5767-5780.
Puthussery, J.V., Singh, A., Rai, P., Bhattu, D., Kumar, V., Vats, P., Furger, M., Rastogi, N., Slowik, J.G., Ganguly, D., Prevot, A.S.H, Tripathi, S.N., Verma, V. Real-Time Measurements of PM2.5 Oxidative Potential using Dithiothreitol (DTT) Assay in Delhi, India, Environmental Science and Technology Letters, https://doi.org/10.1021/acs.estlett.0c00342
Yu, H, Puthussery, J.V., Verma, V., 2019. A semi-automated multi-endpoint reactive oxygen species activity analyzer (SAMERA) for measuring the oxidative potential of ambient PM2.5 aqueous extracts, Aerosol Science and Technology, 54 (3), 304320.
Salana, S., Wang, Y., Puthussery, J.V., Verma, V., 2021. A Semi-automated instrument for cellular oxidative potential evaluation (SCOPE) of water-soluble extracts of ambient particulate matter. Atmospheric Measurement Techniques, 14, 7579-7593.